摘要
目的检测慢性乙型肝炎(CHB)患者血清中可溶性可诱导共刺激分子(s ICOS)和可溶性程序性死亡蛋白1(s PD-1)的蛋白水平,并初步探讨其意义。方法收集80例CHB患者和30例健康对照者外周血单个核细胞(PBMCs)和血液上清。根据HBs Ag和HBe Ag测定值,将CHB患者分为HBe Ag(-)和HBe Ag(+)两组;并将HBe Ag(+)组分为免疫耐受期和免疫清除期。用ELISA法检测以上研究对象外周血上清中s ICOS和s PD-1蛋白浓度。用实时荧光定量PCR检测PBMCs中ICOS、PD-1和PD-1Δex3的表达。结果与对照相比,s ICOS在CHB患者中均增高(P<0.05);而s PD-1仅在HBe Ag(+)组显著增高(P<0.01),其中免疫清除期比免疫耐受期患者增高更明显(P<0.05)。CHB患者的s PD-1与AST呈正相关(P<0.01);且HBe Ag(+)组的s PD-1与ALT呈正相关(P<0.01);CHB患者的s ICOS与HBV-DNA呈弱的负相关性(P<0.05)。活化前,两组CHB患者的ICOS mRNA表达水平均降低(P<0.05)。结论 s ICOS和s PD-1在CHB患者的血清中均异常增高,提示s ICOS和s PD-1可能参与了CHB的发生发展。
Objective To detect the level of soluble inducible co-stimulator molecule (sICOS) and soluble programmed death protein 1 (sPD-1) in peripheral blood supematant in patients with chronic hepatitis B and then discuss its significance with other index.Methods Eighty patients with CHB and thirty healthy persons were enrolled in our study.the peripheral blood supernatants were collected and isolated the peripheral blood mononuclear cells (PBMCs) in CHB patients.According to the clinical HBsAg and HBeAg results,the CHB patients can be divided into the HBeAg(-) group and the HBeAg (+) group,simultaneously,the HBeAg (+) group was divided into two parts:the immune tolerance group and immune clearance group.The sICOS and sPD-1 expression in the peripheral blood was measured by ELISA.The mRNA expression of ICOS,PD-1 and PD-1Aex3 in PBMCs of patients with CHB were detected by real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR).Results sICOS level was significantly higher in CHB patients compairing the healthy controls (P <0.05);.as same as the HBeAg(+) persons,sPD-1 was increased(P <0.01),in which the sPD-1 level was increased more obviously in patients who were in immune clearance phase than those in immune tolerance phase (P <0.05).sPD-1 was positively correlated with the AST levels in CHB patients(P <0.01);The sPD-1 was only correlated with the ALT levels in the HBeAg(+) patients (P <0.01).But there is no correlation between sICOS level and ALT or AST levels.A weakly negative correlation between HBV-DNA and sICOS levels was observed (P < 0.05).Whereas the sICOS did not correlate with HBeAg levels.Compared with the healthy controls,before the activation and stimulation,the ICOS mRNA expressions were decreased in two CHB groups (P < 0.05).Conclusions The sICOS and sPD-1 levels are significantly higher in the serum of patients with CHB and they may be able to participate in the development of CHB.
出处
《基础医学与临床》
CSCD
2015年第7期916-920,共5页
Basic and Clinical Medicine
基金
国际科技合作专项项目(2015DFA30420)