摘要
目的探讨过表达钙周期蛋白S100A6对子宫内膜异位症(EMs)在位子宫内膜间质细胞生物学行为的影响。方法体外培养EMs在位子宫内膜间质细胞,将慢病毒载体和表达S100A6的重组慢病毒Lv-S100A6分别处理在位内膜间质细胞,设对照组,用CCK-8法检测细胞增殖,Transwell观察细胞迁移,流式细胞仪观察细胞凋亡。结果 LvS100A6组细胞的增殖活性明显高于干预组以及对照组(P<0.05)。Lv-S100A6组细胞的迁移数明显高于干预组以及对照组(P<0.05)。Lv-S100A6组细胞凋亡率为2.99%,明显低于对照组的13.48%和干预组的14.40%(P<0.05)。结论通过上调细胞中S100A6的表达水平能增加EMs在位子宫内膜间质细胞的增殖能力,促进细胞的迁移并抑制细胞的凋亡。
Objective To investigate the influence of over-expressed S100A6 on proliferation,migration and apoptosis in eutopic endometrial stromal cells.Methods The eutopic endometrial stromal cells were cultivated and divided into Lv-S100A6 group and negative group,which was infected with the recombinant lentivirus S100A6 （LvS100A6） and recombinant lentivirus vector respectively.CCK-8 assay was performed to identify the proliferation of cell.The cell migration was detected by Transwell chamber.Flow cytometry method was performed to detect cell apoptosis.Results As compared with the blank group and negative control group,the proliferation was increased in eutopic endometrial stromal cells after transfection with Lv-S100A6 （P ＜ 0.05）,the ability of migration was promoted.However,the apoptosis rate in eutopic endometrial stromal cells after transfection with Lv-S100A6 （2.99％）was decreased compared with the blank control group（13.48％） and the negative group（14.40％） （P ＜ 0.05）.Conclusions S100A6 can effectively increase the proliferation and migration in eutopic endometrial stromal cells,and inhibit the cell apoptosis.
出处
《基础医学与临床》
CSCD
2015年第7期925-928,共4页
Basic and Clinical Medicine
基金
江西省自然科学基金(20123BCB22010)
