溃疡性结肠炎患者RAGE表达及血清sRAGE水平的临床研究

Clinical study on RAGE expression and serum sRAGE level in patients with ulcerative colitis

目的探讨溃疡性结肠炎(UC))患者糖基化终末产物受体(RAGE)的表达、血清可溶性糖基化终末产物受体(sRAGE)水平及其临床意义。方法 2012年3月至2014年3月收治的36例UC患者为UC组(活动期14例,缓解期22例),20例健康体检者为健康对照组,20例结肠镜检查肠黏膜正常者为正常对照组,20例普通结肠炎肠镜检查肠黏膜正常者为疾病对照组。采用酶联免疫吸附法(ELISA)检测各组血清sRAGE、C反应蛋白(CRP)、肿瘤坏死因子-α(TNF-α)水平,免疫组化染色检测结肠黏膜RAGE表达。结果 UC组患者血清sRAGE、CRP、TNF-α水平均高于疾病对照组和健康对照组(P均<0.05),活动期患者的血清sRAGE、CRP水平均高于缓解期(P均<0.05);各组血清中sRAGE水平和CRP、TNF-α水平呈正相关关系(r=0.4936,0.5984;P均<0.05)。UC组病变区的RAGE表达阳性率(86.1%)明显高于正常区(50.0%)及疾病对照组(55.0%)、正常对照组(45.0%),差异均有统计学意义(P均<0.05),但UC组正常区、疾病对照组、正常对照组的结肠黏膜RAGE表达阳性率之间差异均无统计学意义(P均>0.05)。结论 RAGE及sRAGE可能参与了UC的发病,是UC独特的免疫炎症性发病特点的反映。

Objective To investigate the expression of receptor for advanced glycation end products （RAGE） and the level of serum soluble receptor for advanced glycation end products （sRAGE） and the clinical significance in patients with ulcerative colitis （UC）. Methods Thirty-six UC patients （ 14 eases for active stage,22 cases for remission stage） treated in our hospital from March 2012 to March 2014 were collected as UC group;20 healthy subjects were served as health control group ;20 patients with normal colon mucous membrane confirmed by colonoscopy served as normal control group ;20 common colitis patients with normal colon mucous membrane by eolonoscopy were served as disease control group. The levels of serum sRAGE, C-reactive protein （CRP）and tumor necrosis factor-α （TNF-α）were measured by enzyme-linked immunosor- bent assay （ELISA） , and RAGE expression in colon mucous membrane tissues was detected by immunohistoehemical stai- ning. Results The levels of serum sRAGE, CRP and TNF-α in UC group were all significantly higher than those in disease control group and health control group（ all P 〈 0.05 ）. The levels of serum sRAGE and CRP in active stage patients were all significantly higher than those in remission stage patients （ all P 〈 0.05 ）. The sRAGE levels in each group were positively correlated with CRP and TNF-α levels （ r = 0. 4936,0. 5984 ; all P 〈 0.05 ）. The positive expression rate of RAGE in diseased region of UC group （ 86.1% ） was significantly higher than those in normal region of UC group （ 50.0% ） , disease control group （ 55.0% ） and normal control group （45.0%） （ all P 〈 0.05 ）, but there were no statistical differences in positive expression rates of RAGE among normal region of UC group, disease control group and normal control group （ all P 〉 0. 05 ）. Conclusion RAGE and sRAGE might be involved in occurrence of UC and could be a unique reflection of im- mune inflammatory disease characteristics.