影响泽泻蕨孢子培养的环境因素研究

Effects of Environment Factors on Spores Culture of Hemionitis arifolia Moore.

为了解环境因子对泽泻蕨（Hemionitis arifolia Moore.）培养的影响,采用孢子培养技术研究温度、光照、pH、密度等对其有性繁殖的影响.结果表明,泽泻蕨孢子为需光萌发,孢子萌发和配子体发育的最适环境条件为：温度25℃,日光灯光照强度为150 μmol m^–2s^–1,pH 为6.5,播种密度为5-8 grains cm^–2,幼苗管护简单,成活率较高,未见病虫害.这为保护和开发我国泽泻蕨资源提供了理论依据和技术支持.

In order to understand the inlfuence of environment factors on culture of Hemionitis arifolia Moore., the effects of temperature, light condition, pH and density on sexual reproduction of spores were studied by micro culture. The results showed that the germination of H. arifolia spores need light, the optimum condition for spore germination and gametophyte development of H. arifolia was under 25℃, 150 μmol m–2s–1 of light intensity, pH=6.5 with sowing density of 5–8 grains cm–2. The management of seedlings was simple. The survival of H. arifolia was high with few disease and insect pest. So, these would provide the technical assistance and theoretical foundation for protection and development of H. arifolia resources.