HLA-DP、C基因多态性与新疆维吾尔族HBV感染的相关性研究

Association of human leukocyte antigen variants with hepatitis B virus infection in Uygurs population in China

目的探讨人类白细胞抗原(HLA)基因多态性与新疆维吾尔族人群乙型肝炎病毒(HBV)感染后病毒自然清除的关联。方法收集新疆维族HBV持续感染者245例(128例慢性乙肝患者,117例乙肝无症状携带者)和150例HBV感染自发清除者(对照组),对HLA基因2个位点和UBE基因1个位点进行测序分析,比较HBV组与对照组HLA基因型频率和等位基因频率的差异。结果1 HLA-DP rs9277535AA型频率在HBV组中明显少于对照组(P=0.023),G等位基因在两组间差异有统计学意义(P=0.020);HLA-C rs3130542GG型和A等位基因在HBV组和对照组中差异有统计学意义(P<0.001);UBE2L3 rs4821116基因型频率和等位基因频率在两组间差异无统计学意义(P>0.05);2 rs9277535A和rs3130542G构成了相对降低HBV感染风险的单倍型,rs9277535G和rs3130542A构成了相对提高HBV感染风险的单倍型;3维吾尔族人群HLA保护性等位基因频率高于汉族和藏族人群。结论 HLA-DP rs9277535、HLA-C rs3130542多态性与新疆维吾尔族人群HBV感染后结局有关;HLA保护性等位基因频率越高的人群,其HBV感染后的自然清除率越高。

Objective To invest the associations of human leukocyte antigen polymorphism with the HBV viral clearance in Uygur population of Xinjiang. Methods 245 Uygur HBV carriers（128 chronic HBV carriers and 117 〈br〉 inactive carriers） and 150 Uygur controls （ naturally cleared the HBV virus） were recruited. Three single nucleotide polymorphisms （SNP） were genotyped. Results ①The frequency of HLA-DP rs9277535AA type in HBV carriers group was significantly lower than controls （ P=0. 023 ） . The frequency of A allele distribution in controls was sig-nificantly higher than in carriers （ P=0. 020 ） . The frequency of HLA-C rs3130542 GG type and A allele were dif-ferent between HBV carriers and controls（P〈0. 001）. The frequency of UBE2L3 rs4821116AA type and G allele were not significantly different between HBV carriers and controls （ P 〉0. 05 ） .② We found that rs9277535 and rs3130542 were in linkage disequilibrium,and the rs9277535A/rs3130542G type was a possible protective factor of HBV,while rs9277535G/rs3130542A type in the subjects was a relative risk. Conclusion HLA-DP rs9277535, HLA-C rs3130542 gene polymorphisms are associated with the outcomes of hepatitis B virus infection in Uygur pop-ulation of Xinjiang in China. The more frequency of HLA protective alleles, the higher rate of HBV natural clear-ance.