实验室检测埃博拉病毒对诊疗的临床意义

Laboratory tests for clinical diagnosis and treatment of Ebola virus disease

目的分析实验室PCR检测结果对埃博拉病毒病患者诊疗的临床意义。方法在2014年11月14日至2015年3月14日期间,利比里亚中国埃博拉治疗中心(Ebola Treatment Unit,ETU)共收治住院患者101例,送检172份标本,通过实验室实时荧光PCR检测病毒核酸及CT值,分析实验室检测结果与病情变化及预后的关系。结果共有10例埃博拉病毒核酸阳性患者,死亡4例,治愈6例。4例死亡病例中,病毒核酸PCR CT值均较低,表明其体内病毒载量较高;在6例治愈病例中,随着病情的好转,病毒核酸PCR CT值逐渐升高,表明病毒载量逐渐降低。结论实验室实时荧光PCR检测病毒核酸及其CT值可为临床诊断、治疗提供指导。

Objective To analyze the clinical significance of PCR results for diagnosis and treatment of Ebola virus disease.Methods From 2014 November to 2015 March,a total of 101 patients were admitted to China Ebola Treatment Unit（ETU）in Liberia,and 172 specimens were sent for laboratory test：detection of Ebola virus RNA using real time PCR.The result of detection was recorded as positive or negative.If positive,the cycle threshold（CT）in real-time PCR was also reported.The relationship of laboratory findings with illness and prognosis was analyzed.Results Ten patients were identified as virus positive.The results of the first detection were positive in 8patients and negative in 2patients,and the 2patients were positive in a second detection performed again 48 hlater.The low CT values of PCR test observed in the 4patients suggested high virus loads,and they died finally.In the 6survived patients the CT values increased as their condition became better,which indicated that the virus loads decreased gradually.Conclusion Detection of Ebola virus mRNA by real-time PCR and the CT value can provide important reference for diagnosis and treatment of Ebola virus disease.