重组可溶性人 CD40L 诱导人脐静脉内皮细胞损伤

Injury effect of recombinant soluble human CD40 ligand on human umbilical vein endothelial cells

目的：探讨重组可溶性人CD40L（ rshCD40L）对人脐静脉内皮细胞（ HUVECs）的损伤作用及其在动脉粥样硬化中的作用。方法：应用rshCD40L刺激人脐静脉内皮细胞12 h；MTS法观察HUVECs的生存活性， ELISA法测内皮细胞E－选择素（ E－selectin）、细胞间黏附分子（ ICAM）－1、组织因子（ TF）、组织因子途径抑制物（ TF－PI）表达的变化，比色法测脂质过氧化物丙二醛（ MDA）含量及超氧化物歧化酶（ SOD）活力。结果：与正常组比较，不同浓度的rshCD40L（0．5、1、2、3 mg／L）对内皮细胞的生存活性无明显影响；0．5 mg／L rshCD40L即可增加内皮细胞E－selectin、sICAM－1、TF、TFPI的分泌，差异有统计学意义（ P＜0．01），同时增加内皮细胞MDA的含量、降低SOD活性（P＜0．05）。结论：0．5～3 mg／L rshCD40L对内皮细胞生存活性无明显影响，但已经引起内皮细胞功能障碍，增加内皮细胞炎症和外源性凝血反应，诱导内皮细胞脂质过氧化物损，使其抗氧化能力下降。

AIM：To investigate the damage in human umbilical vein endothelial cells （ HUVECs） induced by recombinant soluble human CD40 ligand （rshCD40L）.METHODS：The cultured HUVECs were treated with rshCD40L for 12 h.The survival activity of the HUVECs was observed by MTS assay.The expression of E-selectin, intercellular ad-hesion molecule （ICAM）-1, tissue factor （TF） and tissue factor pathway inhibitor （TFPI） was measured by ELISA.The activity of superoxide dismutase （ SOD） and the level of malondialdehyde （ MDA） were detected by the methods of thibabi-turic acid （TBA） .RESULTS：Compared with normal group, different concentrations of rshCD40L （0.5, 1, 2, 3 mg/L） had no obvious effect on the survival activity of the HUVECs （P〉0.05）.rshCD40L at concentration of 0.5 mg/L promo-ted the secretion of E-selectin, sICAM-1, TF and TFPI in the HUVECs （P〈0.01）.rshCD40L at concentration of 0.5 mg/L also increased MDA content and reduced the activity of SOD in the HUVECs （P〈0.05）.CONCLUSION：0.5～3mg/L rshCD40L has no obvious effect on endothelial cell survival, but already causes endothelial dysfunction by increas-ing endothelial inflammation and exogenous coagulation reaction, inducing lipid peroxides injury and reducing antioxidant capacity.