雷公藤红素抑制食管癌细胞转移及其机制

Anti-metastasis of celastrol on esophageal cancer cells and its mechanism

雷公藤红素是从传统中药雷公藤中提取分离得到的一种醌甲基三萜,具有抗炎、免疫抑制及抗肿瘤等药理活性。本文探讨雷公藤红素对食管癌细胞粘附、迁移及侵袭的影响。用MTT法检测不同浓度的雷公藤红素对食管癌细胞ECA-109粘附的影响;用划痕实验测定不同浓度雷公藤红素对ECA-109细胞迁移的影响;用Transwell实验测定不同浓度雷公藤红素对ECA-109细胞侵袭的影响;用实时荧光定量PCR及Western blot实验检测不同浓度的雷公藤红素对ECA-109中整联蛋白家族及Wnt信号通路的影响。结果显示:雷公藤红素以剂量依赖的方式抑制ECA-109的粘附、迁移及侵袭,抑制整联蛋白β1、β4、αv基因和蛋白的表达,并抑制Wnt信号通路中β-Catenin、LRP6蛋白的表达。研究结果表明雷公藤红素能通过抑制Wnt信号通路和整联蛋白的表达来抑制食管癌细胞的转移。

Celastrol is a quinone methyl terpene extracted from the traditional Chinese medicine Tripterygium wilfordii, which has anti-inflammatory, immune suppression and pharmacological activities, as well as anti-tumor activity. The effects of celastrol on adhesion, migration and invasion of esophageal cancer cells were investigated in this experiment. Human esophageal cancer cell line ECA-109 was used. The inhibition of ECA-109 cells＇ adhesion induced by celastrol was measured by MTT test. The inhibition of ECA-109 cells＇ migration induced by celastrol was measured by scratch test. The invasion inhibition of ECA-109 cells induced by celastrol was measured by Transwell experiment. Quantitative real-time PCR and Western blot were used to determine the effects of different concentrations of celastrol on integrin family and Wnt signaling pathway in ECA-109 cells. The results showed that celastrol inhibited adhesion, migration and invasion of ECA-109 cells and expressions of integrins β1, β4, αv and β-Catenin, LRP6 in Wnt signal pathway in a dose-dependent manner. Therefore the study suggests that celastrol could inhibit the cell metastasis of esophageal cancer by inhibiting the Wnt signaling pathway and the expressions of integrins.