维药异常黑胆质成熟剂对TGF-β_1诱导增生性瘢痕成纤维细胞的影响

Effect of uighur medicine abnormal savda munziq on the TGF-β_1 induced in hypertrophic scar fibroblasts

目的:研究维药异常黑胆质成熟剂(abnormal savda munziq,ASMq)对TGF-β1诱导增生性瘢痕成纤维细胞的影响。方法:组织块法体外培养人增生性瘢痕成纤维细胞,将其分为6组:对照组:加入等体积的培养液;实验组:加或不加不同浓度ASMq(0.2mg/ml,0.4mg/ml,0.6mg/ml,1.0mg/ml)及浓度为5ng/ml的TGF-β1;CCK-8法检测成纤维细胞增殖情况,应用western blot,RT-PCR检测CollagenⅠ及Smad7表达情况。结果:ASMq降低了TGF-β1诱导的成纤维细胞增殖(P<0.05);ASMq下调了TGF-β1诱导的成纤维细胞CollagenⅠm RNA及CollagenⅠ的表达(P<0.05),同时,ASMq明显增加了被TGF-β1抑制的Smad7m RNA和Smad7的表达(P<0.05)。结论:研究结果表明ASMq通过促进Smad7合成来阻断增生性瘢痕成纤维细胞TGF-β/Smad通路信号转导,从而降低成纤维细胞增殖及CollagenⅠ合成。

Objective To investigate the effect of uighur medicine abnormal savda munziq on the TGF-β1induced in hypertrophic scar fibroblasts. Methods Using tissue explant cultures of human hypertrophic scar fibroblasts, divided into six group：control group：add an equal volume of culture medium;and experimental group：treated with TGF-β1with or without different concentration of ASMq（0.2 mg/ml,0.4 mg/ml,0.6 mg/ml,1.0mg/ml）.CCK-8 assay was adopted to detect the proliferation of fibroblasts.western blot and RT- PCR were performed to detect the expression of Collagen Ⅰ and Smad7. Results ASMq reduced the TGF- β1induced in fibroblasts proliferation（P〈0.05）;ASMq down-regulation the fibroblast expression of CollagenⅠ m RNA and CollagenⅠ induced by TGF-β1（P〈0.05）,In addition,ASMq significantly increased the expression of Smad7 m RNA and Smad7 which was suppressed by TGF-β1（P〈0.05）. Conclusions Our results demonstrate that ASMq blocks the TGF- β/Smad signalling pathway in hypertrophic scar fibroblast by up regulation of Smad7,thereby reducing the proliferation of fibroblasts and synthesis of CollagenⅠ.