细胞外基质因子在兔软骨细胞损伤模型中的表达变化

Gene expression of extracellular matrix factors in scratch-wound model of articular chondrocyte in rabbit

目的：研究细胞外基质因子在兔关节软骨细胞损伤后不同时间点的表达变化水平。方法：通过机械划伤制备关节软骨细胞损伤模型,观察软骨细胞在划伤后的形态变化。采用实时荧光定量 PCR 检测软骨细胞在划伤前与划伤后1、3、5 d和7 d 共5个时间点的基质金属蛋白酶13（MMP13）及基质金属蛋白酶抑制物-1（TIMP1）、透明质酸和Ⅱ型胶原（Col-Ⅱ）基因 mRNA 表达水平。结果：成功建立了兔关节软骨细胞损伤模型。与划伤前相比,MMP13基因水平在细胞损伤第1天表达明显下降,随后出现升高趋势,第5天达到最高后逐渐下降。TIMP1基因表达量损伤后先上升至第1天然后下降,在第3天降至最低,随后呈现升高趋势。透明质酸和 Col-Ⅱ基因水平第1天表达明显降低,随后呈上升趋势,透明质酸mRNA 表达量至第5天达到最高,然后逐渐下降。结论：MMP13、TIMP1、透明质酸和 Col-Ⅱ基因在细胞损伤后的表达规律相异,为软骨细胞外基质因子表达与软骨细胞之间关系提供实验依据。

Obj ective ：To explore the expressions of extracellular matrix factor genes in articular chondrocyte of rabbits at different time points after injury.Methods ：The scratch-wound model of chondrocytes was established by mechanical injury, and morphological characteristics of chondrocytes were observed.The mRNA expressions of collagen type Ⅱ （Col-Ⅱ ）, matrix metalloproteinase 1 3 （MMP1 3）,tissue inhibitor of metalloproteinase 1（TIMP1）and hyaluronic acid genes in articular chondrocytes were detected by real-time quantitative polymerase chain reaction （qPCR）in normal and post-injury 1 ,3,5 and 7 day.Results ：The injury model of rabbit articular chondrocyte was successfully established.Compared with the normal cell, the level of MMP1 3 mRNA decreased after 1 day of injury,then increased,reached the peak at 5 days after injury,and then decreased again.The TIMP1 mRNA expression increased at 1 day after injury,then decreased to the minimum at day 3,and then increased.The levels of hyaluronic acid and Col-Ⅱ mRNA were decreased at first,then showed a rising trend. Conclusion：The expression patterns of MMP1 3,TIMP1 ,hyaluronic acid and Col-Ⅱ mRNA are different after articular chondrocyte injury,which suggests extracellular matrix may play a role in articular cartilage injury.