诃子水提物对肺癌A549细胞中p53表达的影响

Expression of p53 in A549 cells treated by aqueous extract of terminalia

目的：探讨诃子水提物对人肺癌 A549细胞增殖的影响及 p53在此过程中的作用。方法：以不同浓度的诃子水提物干预人肺癌 A549细胞为实验组，未经干预的为空白对照组。采用 MTT 法检测10、1、0.1、0.01μg/ml 诃子水提物分别作用24、48、72 h A549细胞后的增殖抑制率；Real-time PCR 法检测 p53在不同浓度诃子水提物作用前后的相对表达量的变化；免疫细胞荧光法检测 p53蛋白的表达。结果：MTT 法检测结果显示，增加水提物浓度，抑制率随之上升；PCR 法检测显示，实验组较对照组 p53的 mRNA 表达量上调；免疫细胞荧光法检测显示，实验组较对照组 p53蛋白表达增多，并与水提物浓度成正比。结论：诃子水提物能诱导 A549细胞凋亡，可能与 p53基因被激活有关。

Obj ective ： To investigate the effects of aqueous extract of terminalion on A5 4 9 human lung cancer cell proliferation,and the possible role of p5 3 in the process.Methods ：Different concentrations of aqueous extract of terminalia were used to treat human lung cancer A5 4 9 cells in different experimental groups.Proliferation inhibition rate was detected by MTT assay at 2 4 ,4 8 and 7 2 h with different concentrations （1 0 ,1 ,0.1 ,0.0 1 μg/ml）aqueous extract of terminalia.p5 3 mPNA expression level was detected by real-time PCR assay;p5 3 protein expression was detected by immunofluorescence assay.Results ：MTT assay showed that the proliferation rate of A5 4 9 cells treated by aqueous extract of terminalia was gradually increased.p5 3 expression in protein and mRNA level were higher in experimental group than that in control group. Conclusion：Aqueous extract of terminalia can induce the apoptosis of A5 4 9 cells,and this process is related to the activation of p5 3 gene.