龙血竭栓对兔直肠破损黏膜刺激性和对豚鼠皮肤致敏性实验

Stimulation of long xue jie’s suppository in rabbits’ damaged rectum mucous membrane and sensitization in guinea pig

目的：观察龙血竭栓的刺激性与致敏性。方法：以龙血竭栓对新西兰兔直肠破损黏膜单次及多次给药，观察其对兔直肠破损黏膜的刺激性；采用 Buehler 法对豚鼠皮肤给药，观察龙血竭栓对其致敏性。结果：新西兰兔直肠破损黏膜单次和多次给药，各剂量组动物给药后未见疼痛症状，粪便未见明显异常，肛门区域和肛门括约肌局部组织未见充血、水肿等现象，直肠破损黏膜未发现药物相关的明显组织病理学改变。Buehler 实验龙血竭栓组动物过敏反应发生率为0%，实验期间未见任何过敏性反应症状。结论：龙血竭栓对兔直肠破损黏膜无刺激性，对豚鼠无致敏作用。

Obj ective ：To evaluate the stimulation and the sensitization of the suppository of Long Xue Jie.Methods ：The suppository of Long Xue Jie was used on New-Zealand rabbits after single and multiple times to evaluate the stimulatory effects of the drug on damaged rectummucous membrane.We used guinea pigs to proceed the skin drug administration to observe the sensitization of long xue jie’s suppository in Buehler test.Results ：There were no painful symptoms in all groups of the New-Zealand rabbits after single and repeated dose in damaged rectum mucous membrane,and the excrements were normal.There were no signs of congestion and edema around the anus and the anus sphincter.Histopathologic examination showed that there were no obvious pathological changes in damaged rectum mucous membrane from the drug.In Buehler test, the sensitization response rate of the dosage group was 0%, and no anaphylactic reaction happened during the test. Conclusion：There is no stimulatory effect of the suppository of Long Xue Jie on damaged rectum mucous membrane in New-Zealand rabbits.The administration of the suppository of Long Xue Jie has no sensitization reaction on guinea pigs.