丙烯酰胺暴露对大鼠神经行为和神经电生理变化的影响

Effect of acrylamide on the neurobehavioral and neurophysiology in rats

目的观察丙烯酰胺暴露对大鼠神经行为和神经电生理变化的影响。方法 SD大鼠30只,随机分为对照组、低剂量染毒组和高剂量染毒组,每组10只。低剂量染毒组和高剂量染毒组分别以20 mg/kg和40 mg/kg丙烯酰胺行腹腔注射,每天1次,每周3次,共染毒10周。观察大鼠的一般状态、体重和步态变化,并检测各组大鼠的神经传导速度和神经传导潜伏期。结果与对照组相比,丙烯酰胺染毒大鼠的体重增长缓慢,高剂量染毒组大鼠8周后出现体重负增长。低剂量染毒组和高剂量染毒组大鼠分别在8周、4周出现周围神经病特有的异常步态,到10周时异常步态加重,其神经行为评分值分别为2.00±0.00和4.00±0.00,明显高于对照组(P<0.05)。神经电生理结果显示,染毒3周大鼠开始出现神经传导速度降低和神经传导潜伏期延长,至10周时,低剂量染毒组和高剂量染毒组大鼠神经传导速度分别降低15.13%和33.06%,神经传导潜伏期分别延长17.41%和58.75%,与对照组比较,差异均有显著性意义(P<0.05)。结论丙烯酰胺腹腔注射能导致大鼠的神经行为和神经电生理异常变化,可诱导大鼠周围神经病。

Objective To observe the effect of acrylamide on the neurobehavioral and neurophysiology of rats. Methods Thirty rats were randomly and evenly divided into 3 groups： control group,low- and high- dose group. The rats in 3groups were injected intraperitoneally with normal saline,20 mg / kg and 40 mg / kg acrylamide respectively for 10 weeks（ 3d / w）. The general state,gait and weight changes of the rats were observed,and nerve conduction velocity and distal latency were detected. Results Compared with the control group,the weight of rats exposed to acrylamide decreased progressively with prolonged exposure. After 8 weeks,there was weight loss in the rats of high- dose group. In rats of low- and high- dose group,abnormal gait were found on 8 w and 4 w respectively and the gait scores were 2. 00 ± 0. 00 and 4. 00 ±0. 00 respectively at 10 weeks. From the 3rd week,distal latency increased and nerve conduction velocity decreased significantly. Compared with the control group,nerve conduction velocity were increased by 15. 13% and 33. 06%,and distal latency decreased by 17. 41% and 58. 75% in the low- and high- dose groups（ P〈0. 05）. Conclusion The chronic exposure to acrylamide would cause the abnormal changes of neurobehavioral and neurophysiological in rats,then lead to peripheral neuropathy.