不同浓度艾烟干预对大鼠肺脏及血清SOD、MDA表达的影响

Effects of moxa smoke with different concentrations on expression of SOD and MDA in lung and serum of rats

目的:观察不同浓度艾烟干预对雄性大鼠血清及肺脏组织中超氧化物歧化酶(SOD)、丙二醛(MDA)的影响,探讨艾烟干预的安全性浓度。方法:将Wistar雄性大鼠32只随机分成4组,即对照组、低浓度组、中浓度组、高浓度组,每组8只。4组大鼠分别暴露于全自动动式染毒柜中,艾烟遮光度分别设为0%、10%、40%、70%,每天20min,26周后检测大鼠血清、肺组织及支气管肺泡灌洗液中SOD活力和MDA含量。结果:与对照组比较,高浓度组大鼠血清中SOD活力下降(P<0.05),低浓度组、中浓度组差异均无统计学意义(均P>0.05),血清中MDA含量艾烟低浓度组、中浓度组、高浓度组均较对照组增加,但差异无统计学意义(均P>0.05);肺组织及支气管肺泡灌洗液中SOD活力和MDA含量在艾烟干预各组间比较差异均无统计学意义(均P>0.05)。结论:低、中浓度组未见显著毒性反应,较为安全;高浓度组长期暴露损伤机体抗氧化能力,可造成氧化损伤。

Objective To observe the effects of intervention of moxa smoke with different concentrations on su- peroxide dismutase （SOD） and malondialdehyde （MDA） in serum and lung of male rats, so as to explore the safety concentration of moxa smoke. Methods A total of 32 Wistar male rats were randomly divided into a control group, a low-concentration group, a moderate-concentration group and a high-concentratlon group, 8 rats in each one. All the rats were exposed in the full-automatic toxicant exposure cabinet, and the overshadow of moxa smoke was set at 0%, 10%, 40% and 70%, respectively. Each rat was exposed for 20min per day. After 26 weeks, the activities of SOD and content of MDA in serum, lung organ and bronchoalveolar lavage fluid were tested. Results Compared with the control group, the activities of serum SOD in the high-concentration group were reduced （P〈 0.05）, but those in the low-concentration group and moderate-concentration group were not significantly different （both P〉0.05）. Compared with the control group, the content of serum MDA in the low-concentration group, moderate-concentration group and high-concentration group was increased insignificantly （all P〈 0.05）. There were no significant differences regarding activities of SOD and content of MDA in lung organ and bronchoalveolar lavage fluid among each moxa smoke group （all P〉0.05）. Conclusion There is no obvious toxic reaction in the low-concentration group and moderate-concentration group; in the high-concentration group the antioxidant ability is damaged due to long-term exposure.