电针对帕金森病模型大鼠纹状体相关蛋白mRNA表达的影响

Effects of electroacupuncture on the expression of related protein mRNA in the striatum in rats with Parkinson's disease

目的:探讨电针治疗帕金森病(PD)的作用机制。方法:将40只健康雄性SD大鼠随机分为正常组、假手术组、模型组、电针组,每组10只。用微量注射法向模型组、电针组大鼠右侧纹状体注射6-羟基多巴胺制备偏侧PD大鼠旋转模型,假手术组以0.9%NaCl溶液进行微量注射。正常组、模型组、假手术组不予任何干预;电针组电针"风府""太冲",采用连续波,频率2Hz,强度1mA,持续30min,每日1次,治疗2周。行为学测试检测PD大鼠干预前后旋转行为的改变,采用RT-PCR法检测各组大鼠纹状体部胶质原纤维酸性蛋白(glial fiber acidic protein,GFAP)、缝隙连接蛋白43(connexin 43,Cx43)mRNA的表达。结果:模型组大鼠干预前后旋转行为差异无统计学意义(P>0.05),电针组大鼠干预前后差异有统计学意义(P<0.01);模型组大鼠GFAP mRNA、Cx43mRNA表达较正常组和假手术组高(均P<0.01),经电针治疗后,电针组大鼠GFAP mRNA、Cx43mRNA表达较模型组降低(均P<0.01)。结论:电针防治帕金森病的机制可能与抑制星形胶质细胞的激活有关。

Objective To explore action mechanism of electroacupuncture （EA） on treatment of Parkinson＇s disease （PD）. Methods A total of 40 healthy male SD rats were randomly divided into a normal group, a sham operation group, a model group and an EA group, 10 rats in each group. PD rat model was duplicated by micro injection of 6-hydroxyl dopamine into right striatum of rats, and the rats in the sham operation group were treated with micro injection of 0.9% NaC1. Rats in the normal group, model group and the sham operation group received no treatment; rats in the EA group were treated by EA at ＂Fengfu＂ （GV 16） and ＂Taichong＂ （LR 3） with continuous wave, 2 Hz in frequency, 1 mA in intensity for 30 min. The treatment was given once a day for total 2 weeks. Behavioral test was used to evaluate rotational behavior changes of PD rats. RT-PCR method was applied to detect the expression of GFAP （glial fiber acidic protein） mRNA and Cx43 （connexin 43） mRNA in the striaturn. Results The difference of rotational behavior was not significant before and after treatment in the model group （P〉0.05）, while that in the EA group was significant （P〈0. 01）. The expression of GFAP mRNA and Cx43 mRNA in the model group was significantly higher than that in the normal group and sham operation group （all P〈0.01） ; after EA treatment, the expression of GFAP mRNA and Cx43 mRNA in the EA group was lower significantly than that in the model group （both P〈0.01）. Conclusion The action mechanism of EA for prevention and treatment of Parkinson＇ s disease may be associated with inhibiting the activation of astrocytes.