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人永生化角质形成细胞急性光损伤程度与线粒体膜电位变化的关系

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摘要 目的:探讨人永生化角质形成细胞(Ha Ca T细胞)急性光损伤程度与线粒体膜电位变化的关系。方法:用不同剂量的UVB照射Ha Ca T细胞,通过光学显微镜观察细胞形态学改变,MTT检测细胞增殖活性(吸光度),JC-1荧光染料法检测线粒体膜电位的改变。结果:100 m J/cm2剂量UVB照射可引起Ha Ca T细胞形态发生变化,与空白对照组相比增殖活性降低(P<0.05),线粒体红色/绿色荧光强度值明显低于正常对照组(P<0.05);随着照射剂量增加,红色/绿色荧光强度值逐渐下降。结论:线粒体膜电位的降低可以反映Ha Ca T细胞急性光损伤的程度,线粒体膜电位越低,Ha Ca T细胞急性光损伤的程度越大。
作者 邓蕙妍 张倩雯 刘清 江娜 张尔婷 李润祥 梁碧华 朱慧兰
机构地区 广州市皮肤病防治所 广州医科大学
出处 《皮肤性病诊疗学杂志》 2015年第3期210-213,共4页 Journal of Diagnosis and Therapy on Dermato-venereology
基金 广州市医药卫生科技项目(编号:20141A011071) 广东省科技计划项目(编号:2013B021800044)
关键词 HACAT细胞 UVB 光损伤 线粒体
分类号 R363 [医药卫生—病理学]
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参考文献12

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二级参考文献20

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皮肤性病诊疗学杂志
2015年 第3期

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