亚低温脑内冷诱导RNA结合蛋白的表达特征

Expression features of cold inducible RNA binding protein in rat brains under hypothermia

目的初步检测RNA结合蛋白(CIRP)在低温大鼠模型中,尤其是脑组织中的表达特征。方法大鼠被分为两组:对照组和低温组。低温组大鼠先置于低温下2 h,复温后2 h断头处死。提取总RNA,反转录后得到c DNA,实时多聚酶链反应(RT-PCR)方法检测CIRP在大鼠脑内的表达情况。利用实时PCR定量检测大鼠脑、心脏、肝脏中CIRP mRNA表达。结果常温下CIRP mRNA均能在脑、心、肝组织中检测出。低温情况下,脑组织中下丘脑、皮层和海马均可见CIRP mRNA表达增高,且海马中CIRP mRNA表达增高最显著。定量PCR检测显示低温情况下CIRP mRNA在脑皮层中的表达比对照组高1.5倍。心脏和肝脏中CIRP mRNA的表达在低温时与对照组差别不大。结论低温情况下CIRP mRNA在脑皮层和海马中会明显增高,而肝脏、心脏中CIRP mRNA的表达不受低温影响。提示低温可以使CIRP在神经组织中的表达特异性增高。

Objective The expression of RNA-binding protein （CIRP） in the rat brain under hypothermia is investigated.Methods Rats were divided into two groups：control group under normothermia and experimental group under hypothermia.The rats were decapitated after hypothermia for 2 h.Total RNA was isolated according to the TRIzol method.RNA was reverse transcribed to obtain cDNA.Real-time polymerase chain reaction （RT-PCR） was performed to detect CIRP mRNA expression in the rat brain.A real-time PCR system was used to quantitatively analyze CIRP mRNA expression in the rat brain, heart, and liver.Results CIRP mRNA expression was detected in all brain, heart and liver tissues.Under hypothermic conditions, CIRP mRNA expression was increased in the hypothalamus, cortex, and hippocampus.However, the most obvious increase was observed in the hippocampus. Quantitative analysis demonstrated that hypothermia significantly induced the increased expression of CIRP mRNA in the cortex, compared with the control group （1.5 fold）.However, the expression remained unchanged in the heart and liver.Conclusion CIRP overexpression in the rat, particularly in the hippocampus and cortex, parallels the reduction in hypothermia.Hypothermia, however, does not affect the CIRP mRNA expression in the heart and liver. These results indicate that CIRP may be induced by hypothermia characteristically in the brain tissues.