NLRP7在早期葡萄胎诊断中的应用

The Application of NLRP7 protein Expression in Diagnosis of Early Hydatidiform Mole

目的探讨NLRP7、P57和Ki-67免疫组织化学染色在早期葡萄胎的表达情况及应用价值。方法根据葡萄胎病理学诊断标准结合P57的表达,将确诊的葡萄胎分为部分性(PHM)和完全性葡萄胎(CHM),利用免疫组织化学染色检测妊娠早期的部分性葡萄胎(n=22)和完全性葡萄胎(n=24)石蜡组织切片中NLRP7和Ki-67蛋白的表达。结果 NLRP7蛋白在部分性葡萄胎和完全性葡萄胎的表达率分别为54.55%(12/22)和50%(12/24),差异无统计学意义(P>0.05),但CHM组NLRP7蛋白的表达强度组内比较有统计学差异,且强阳性表达组和部分性葡萄胎组相比,有显著统计学差异(P<0.01)。Ki-67在部分性和完全性葡萄胎组表达率分别为59.09%(13/22)和79.17%(19/24),有统计学差异(P<0.05)。结论 NLRP7蛋白单项检测不可直接用于鉴别部分性和完全性葡萄胎,需进一步检测基因突变,但联合P57和Ki-67蛋白可为复发性葡萄胎的预防及监测提供指导意义。

Objective To investigate the expression of NLRP7, P57 and Ki-67 in hydatidiform mole.Methods Immunohis-tochemistry was used to detect the expression of NLRP 7, P57 and Ki-67 in paraffin-embedded tissue sections of 22 partial hydatidi-formmole （PHM） cases and 24 complete hydatidiformmole （CHM） cases in early trimester of pregnancy.Results Positive staining rates of P57 were 86.36%（19/22） in PHM and 0.0%（0/24） in CHM.There was no statistical difference in the expressions of NL-RP7 between PHM and CHM （P〉0.05）, but the intensity of NLRP7 in PHM was weaker than that in CHM （P〈0.01）.Positive staining rate of Ki-67 was 59.09%（13/22） in PHM and 79.17%（19/24） in CHM respectively.There was no statistical difference in Ki-67 expression including distribution and intensity between PHM and CHM （P〉0.05）.Conc lusion NLRP7 depending on the severity of its mutations was not suitable to distinguish PHM and CHM , however, co-detecting NLRP7, P57 and Ki-67 contributes to understanding and monitoring of recurrent hydatidiform mole .