摘要
目的探讨NLRP7、P57和Ki-67免疫组织化学染色在早期葡萄胎的表达情况及应用价值。方法根据葡萄胎病理学诊断标准结合P57的表达,将确诊的葡萄胎分为部分性(PHM)和完全性葡萄胎(CHM),利用免疫组织化学染色检测妊娠早期的部分性葡萄胎(n=22)和完全性葡萄胎(n=24)石蜡组织切片中NLRP7和Ki-67蛋白的表达。结果 NLRP7蛋白在部分性葡萄胎和完全性葡萄胎的表达率分别为54.55%(12/22)和50%(12/24),差异无统计学意义(P>0.05),但CHM组NLRP7蛋白的表达强度组内比较有统计学差异,且强阳性表达组和部分性葡萄胎组相比,有显著统计学差异(P<0.01)。Ki-67在部分性和完全性葡萄胎组表达率分别为59.09%(13/22)和79.17%(19/24),有统计学差异(P<0.05)。结论 NLRP7蛋白单项检测不可直接用于鉴别部分性和完全性葡萄胎,需进一步检测基因突变,但联合P57和Ki-67蛋白可为复发性葡萄胎的预防及监测提供指导意义。
Objective To investigate the expression of NLRP7, P57 and Ki-67 in hydatidiform mole.Methods Immunohis-tochemistry was used to detect the expression of NLRP 7, P57 and Ki-67 in paraffin-embedded tissue sections of 22 partial hydatidi-formmole (PHM) cases and 24 complete hydatidiformmole (CHM) cases in early trimester of pregnancy.Results Positive staining rates of P57 were 86.36%(19/22) in PHM and 0.0%(0/24) in CHM.There was no statistical difference in the expressions of NL-RP7 between PHM and CHM (P〉0.05), but the intensity of NLRP7 in PHM was weaker than that in CHM (P〈0.01).Positive staining rate of Ki-67 was 59.09%(13/22) in PHM and 79.17%(19/24) in CHM respectively.There was no statistical difference in Ki-67 expression including distribution and intensity between PHM and CHM (P〉0.05).Conc lusion NLRP7 depending on the severity of its mutations was not suitable to distinguish PHM and CHM , however, co-detecting NLRP7, P57 and Ki-67 contributes to understanding and monitoring of recurrent hydatidiform mole .
出处
《牡丹江医学院学报》
2015年第3期11-13,共3页
Journal of Mudanjiang Medical University
基金
东莞市医疗卫生科技计划项目资助
(201210515023170)
湛江市科技计划项目资助(2012C3101011)