摘要
[目的]细菌分离中,梯度稀释及微量点样法可较好地保持细菌的原位丰度,理论上对优势菌的分离有较高的概率;应用梯度稀释及微量点样法分离γ-变形菌是一个有趣的研究点。[方法]应用梯度稀释及微量点样法,结合不同的分离条件,对7种环境样品进行γ-变形菌分离。[结果]从7种环境样品中成功分离52株细菌,经鉴定γ-变形菌为42株,占分离细菌总数的80.77%,呈现明显的γ-变形菌偏好性;已获得的γ-变形菌分属于8个不同的属,优势菌属为Klebsiella、Enterobacter、Escherichia,占已分离γ-变形菌的78.58%。[结论]实验所应用的梯度稀释及微量点样法对γ-变形菌的分离呈明显的偏好性(P<0.05),是环境γ-变形菌分离方法的重要补充,该方法适用于环境优势菌的分离尝试。
[Objective]The gradient dilution and simple drop plate method in bacteria isolation maintains the in situ abundance of cultural bacteria and high separating probability of dominant bacteria; applying such method for environmental γ-proteobacteria isolation is an interesting research point.[Methods]To isolate γ-proteobacteria from 7 kinds of environmental samples using gradient dilution and simple drop plate method combining different separation conditions.[Results]A total of 52 strains were successfully isolated from 7 environmental samples,42 strains were identified as γ-proteobacteria,accounting for 80.77%,showing significant γ-proteobacteria preference and environmental specificity for strains isolation.The acquired γ-proteobacteria belonged to 8 different genera,and were dominated（accounted for 78.58%） by the genera of Klebsiella,Enterobacter,and Escherichia.[Conclusion]The application of the gradient dilution and simple drop plate method in this study showed significant γ-proteobacteria preference（P〈 0.05）,could be applied intensively in isolation of environmental γ-proteobacteria,and could be attempted to separate for dominant bacteria in different the environments.
出处
《生物技术》
CAS
CSCD
北大核心
2015年第3期281-285,300,共6页
Biotechnology
基金
国家自然科学基金项目("中药多糖利用位点的寻找及其与中药止泻复方调节拟杆菌群的碳源机制"
No.81473455)
江西省自然科学基金项目("基于高通量测序技术的参苓白术散治疗AAD病的微生态机制研究"
No.20122BAB205073)
江西省教育厅项目("基于"碳源-拟杆菌菌群平衡"研究六君子汤的健脾机制"
No.GJJ13598)
江西中医药大学校级研究生创新专项资金项目("参岺白术散基于碳源影响肠道拟杆菌治疗AAD的微生态机制"
No.JZYC14A01)资助~~
关键词
γ-变形菌
细菌
分离
鉴定
[Objective]The gradient dilution and simple drop plate method in bacteria isolation maintains the in situ abundance of cultural bacteria and high separating probability of dominant bacteria
applying such method for environmental γ-proteobacteria isolation is an interesting research point.[Methods]To isolate γ-proteobacteria from 7 kinds of environmental samples using gradient dilution and simple drop plate method combining different separation conditions.[Results]A total of 52 strains were successfully isolated from 7 environmental samples,42 strains were identified as γ-proteobacteria,accounting for 80.77%,showing significant γ-proteobacteria preference and environmental specificity for strains isolation.The acquired γ-proteobacteria belonged to 8 different genera,and were dominated（accounted for 78.58%） by the genera of Klebsiella,Enterobacter,and Escherichia.[Conclusion]The application of the gradient dilution and simple drop plate method in this study showed significant γ-proteobacteria preference（P 0.05）,could be applied intensively in isolation of environmental γ-proteobacteria,and could be attempted to separate for dominant bacteria in different the environments.γ- proteobacteria, bacteria, isolation, identification
