2-丁烯醛生产废水对厌氧生物处理的毒性

Toxicity of crotonaldehyde wastewater to anaerobic biological treatment

以厌氧颗粒污泥为受试生物、乙酸钠为底物,研究了2-丁烯醛废水的厌氧处理毒性及污泥胞外聚合物(EPS)的组成变化.结果表明,2-丁烯醛废水COD≤850mg/L时,厌氧颗粒污泥的比产甲烷活性(SMA)几乎不受影响;当废水COD从2125mg/L提高到4249mg/L时,厌氧颗粒污泥的比产甲烷活性(SMA)从70.5m LCH4/(g VSS·d)降低至9.4m LCH4/(g VSS·d);COD为8499mg/L时,厌氧颗粒污泥的SMA仅为4.7m LCH4/(g VSS·d),且废水中有毒物质表现为杀菌性毒素.随着COD升高,EPS(TOC表征)、多聚糖、蛋白质含量呈现先降低后升高趋势.三维荧光光谱结果显示,不同COD条件下EPS荧光峰数量及位置相同,分别为类酪氨酸荧光峰peak A(λex/λem=275nm/305nm)、类色氨酸荧光峰Peak B(λex/λem=275nm/350nm)、辅酶F420贡献的荧光峰Peak C(λex/λem=415nm/470nm)及类富里酸荧光峰Peak D(λex/λem=335nm/450nm),其中荧光峰peak A和peak B峰强度较强.

With anaerobic granular sludge as the test organisms and sodium acetate as substrate, the toxicity of crotonaldehyde wastewater to anaerobic treatment was studied and the influences on the composition of extracellular polymeric substance （EPS） of the granular sludge were investigated. The results showed that when COD of the crotonaldehyde wastewater was less than 850mg/L, it had little effect on the specific methanogenic activity （SMA）. However, the inhibitory impact was serious when COD increased from 2125mg/L to 4249mg/L, with SMA decreasing from 70.5mLCH4/（gVSS·d） to 9.4mLCH4/（gVSS·d）. Futhermore, when COD was as high as 8499mg/L, SMA was merely 4.7mLCHn/（gVSS·d）, which proved crotonaldehyde wastewater manifesting bactericidal toxin. Meanwhile, the changes of EPS of anaerobic granular sludge under different concentratios of crotonaldehyde wastewater were studied. It displayed that with COD increasing, the amount （represented by TOC）, polysaccharides content and protein content of ESP all exhibited a treand of decrease first and then increase. Moreover, the spectral information about the chemical compositions of EPS was presented by the three-dimensional emission and excitation matrixes （EEMs）. Although similar patterns with peaks numbers and locations were found under various COD conditions, the fluorescence intensities were quite different among the matrixes. Four distinct fluorescence peaks were respectively identified as tyrosine-like peak A （λex/λem=275nm/305nm）, tryptophan-like peak B （λex/λem=275nm/350nm）, peak C （λex/λem=415nm/470nm） contributed by F4z0and fulvic-acid-like peak D （λex/λem=335nm/450nm）, in which the peak intensities of peak A and peak B were stronger than the others.