无机盐和维生素对固体培养槐耳菌丝生长影响的研究

Effect of inorganic salts and vitamin on mycelial growth of Trametes robiniophila Murr. in solid substratum

目的研究固体培养基中无机盐和维生素对槐耳菌丝生长的影响。方法以菌丝生长速度与生长状况为衡量指标,设计单因素和正交试验,筛选KH2PO4、Mg SO4和VB1的最佳添加量。结果槐耳菌丝在KH2PO4 3.0 g·L-1、Mg SO4 2.0 g·L-1、VB1 0.009 g·L-1的培养基上生长速度最大、生长状况最优。结论在固体培养基中加入适量的KH2PO4、Mg SO4和VB1,对槐耳菌丝体生长有一定的促进作用。

Objective To determine the effect of inorganic salts and vitamin on the growth of Trametes robiniophila Murr. mycelial. Methods With the growth rate and growth vigor as the indexes, single factor test and orthogonal test were used to screen the optimal addition amount of KH2PO4, Mg SO4 and VB1. Results Trametes robiniophila Murr. mycelial in the solid substratum with KH2PO4 3.0 g·L^- 1,MgSO4 2.0 g·L^- 1 and VB1 0.009 g·L^- 1 had the optimal growth rate and growth status. Conclusion Adding moderate amount of KH2PO4, MgSO4 and VB1 to solid substratum can partly promote the growth of Trametes robiniophila Murr. mycelial.