CK19、TPO、Galectin-3与HBME-1蛋白表达对甲状腺肿瘤病理诊断作用分析

Analysis of effects by CK19, TPO, galectin-3 and HBME-1 protein expression for pathological diagnosis of thyroid tumor

目的探讨甲状腺病变中的细胞角蛋白19(CK19)、甲状腺过氧化物酶(TPO)、半乳糖凝集素-3(Galectin-3)与人骨髓内皮细胞标记物(HBME-1)表达及其对甲状腺肿瘤病理诊断的价值。方法收集134例甲状腺癌(123例乳头状癌和11例滤泡癌)、34例甲状腺腺瘤、20例结节性甲状腺腺肿以及10例桥本甲状腺炎患者的病理标本,检测其CK19、TPO、Galectin-3与HBME-1的表达情况。结果 CK19、TPO、Galectin-3与HBME-1在甲状腺肿瘤良性组织和恶性组织中的阳性表达率比较,差异有统计学意义(P<0.05);四项指标联合检测对于甲状腺肿瘤良恶性诊断的敏感度和特异度分别为96.3%和100.0%。结论甲状腺恶性肿瘤中CK19、Galectin-3与HBME-1阳性表达增高,而TPO表达缺失,四项指标联合检测对于甲状腺肿瘤良恶性的诊断具有重要价值。

Objective To investigate the expressions of cytokeratin 19（CK19）, thyroid peroxidase（TPO）, Galectin-3, and human bone marrow endothelial cell markers（HBME-1） in thyroid lesions and their effects for pathological diagnosis of thyroid tumor. Methods Pathological samples were collected in 134 cases of thyroid cancer（123 cases of papillary carcinoma and 11 cases of follicular carcinoma）, 34 cases of thyroid adenoma, 20 cases of nodositas goiter and 10 cases of Hashimoto thyroiditis. Their expressions of CK19, TPO, Galectin-3, and HBME-1 were detected. Results The difference of CK19, TPO, Galectin-3, and HBME-1 expressions between benign and malignant tissues in thyroid tumor had statistical significance（P〈0.05）. Combined detection of four indicators had the sensitivity and specificity as 96.3% and 100.0% for diagnosis of benign and malignant thyroid tumor. Conclusion In malignant thyroid tumor, the positive expressions of CK19, Galectin-3, and HBME-1 were increased, while that of TPO was deficient. Combined detection of four indicators provides important diagnostic value for benign and malignant thyroid tumor.