“消银解毒饮”对银屑病血热证外周血淋巴细胞调控作用研究

Study on Regulation Role of Xiaoyinjiedu Drink on Peripheral Blood T-lymphocytes in Blood-heat Syndrome of Psoriasis Patients

目的:观察正常人及银屑病患者应用消银解毒饮治疗前后外周血T淋巴细胞及共刺激因子表达的变化,探讨"消银解毒饮"对银屑病血热证外周血淋巴细胞调控作用。方法:治疗组给予连续8周消银解毒饮治疗,用流式细胞术检测,与对照组相互比较外周血T细胞亚群及共同刺激分子CD28、CD80、CD86的表达情况。结果:治疗组治疗后皮损评分降低;治疗前后与正常人相比CD3+、CD4+细胞及CD4+/CD8+降低,CD8+细胞升高;CD28疗前高于、疗后低于正常组;CD86治疗前后均明显高于正常组;CD80正常组与治疗前均无明显统计学意义,治疗后有下降的趋势。治疗组治疗前、后外周血T细胞亚群CD3+、CD4+、CD8+、CD4+/CD8+和共刺激因子CD28、CD80、CD86均无明显的统计学意义。结论:消银解毒饮临床疗效肯定;银屑病患者外周血T细胞亚群及共刺激因子的表达存在异常;患者临床症状的好转与外周血T细胞亚群及共刺激因子表达的改变是否平行相关尚不确定。

Objective： To explore the regulation role of Xiaoyinjiedu drink on peripheral blood T-lymphocytes in blood-heat syndrome of Ps（ Psoriasis） patients by observing peripheral blood T lymphocytes and the expression of co-stimulatory factor changes of normal and Psoriasis patients,who received Xiaoyinjiedu Drink. Methods： Treatment group was given 8 weeks of medication,using flow cytometry,while comparison was made to the control group in terms of Peripheral Blood T-lymphocytes and Co-stimulatory factor CD28,CD80,CD86. Results： The treatment group＇s lesion scores decreased after treatment; CD3＋,CD4＋and CD4＋/ CD8＋is reduced when compared with normal group before and after treatment; CD28 was higher than the normal group before treatment,but lower than normal group after treatment; CD86 was significantly higher than the normal group before and after treatment; There was no significant difference between the normal group and treatment before,but after treatment there was a downward trend in CD80. There was no significant difference in the peripheral T-lymphocytes CD3＋,CD4＋,CD8＋,CD4＋/ CD8＋and Co-stimulatory factor CD28,CD80,CD86 before and after treatment in the treatment group. Conclusion： Xiaoyinjiedu Drink has positive clinical effect. Psoriasis patients peripheral blood T-lymphocytes and Co-stimulatory factor have abnormal expression. It is uncertain whether the improvement of clinical symptoms of patients and the expression of T-lymphocytes and Co-stimulatory factor are parallel related.