不同剂量郑氏三七散动物肾细胞毒理学研究

Toxicological Experiment Research of the Effect of Different Doses of Zheng's Sanqisan( Notoginseng Powder) on Animal's Renal Cell

目的:通过对大鼠服用不同剂量的郑氏三七散进行干预实验,以此了解郑氏三七散的毒理学性质。方法:实验选取SD雄性大鼠48只,随机分为四组:空白对照组、常规剂量组,中等剂量组和大剂量组,每组12只。干预实验持续3周。实验后抽取各组大鼠的血液检测,称取肾脏重量,留取肾组织标本观察。结果:(1)大鼠给药三周后,不同剂量组以及对照组的大鼠外观特征和行为活动表现正常,各组大鼠体重变化正常;(2)肾脏病理切片显示服用不同剂量郑氏三七散各组大鼠与对照组肾脏被膜完整,肾细胞结构完整,肾组织细胞未见坏死等异常现象出现;(3)各组大鼠肾脏重量及系数均无显著差异;(4)各组大鼠血液中尿素氮(BUN)和肌酐(Cr)无显著差异。结论:本实验条件下,三组不同剂量郑氏三七散使用时均比较安全,没有副作用,适合临床使用。

Objective：To investigate the toxicity of Zheng’s Sanqisan （Notoginseng powder）on rats by taking differ-ent doses of Zheng’s Sanqisan. Method：48 SD rats were randomly assigned to four groups：control group,con-ventional - dose group,medium - dose group and high - dose group,12 rats each group. The experiment lasted for 3 weeks. After the experiment,the testing index was blood urea nitrogen （BUN）and creatinine （Cr）and kidneys of rats. Result：（1）Three weeks of taking the medicine,the rats’appearance and behavior remained normal. There were no significant differences in the weight of rats among different groups. （2）The renal cap-sule of rats in each group was complete and there were no pathological changes among groups. （3）There were no significant changes in renal weights and coefficients of rats among groups. （4）There were no significant differences in blood urea nitrogen and creatinine among different dose groups and control group. Conclusion：No side effect of Zheng’s Sanqisan was found between different dose groups and no significant changes in renal tis-sue cells were witnessed,either. There was no significant toxic difference in renal cell toxicology,therefore, different doses of Zheng’s Sanqisan are suitable for clinical practice.