冬季条件下维生素A水平对蛋鸡抗氧化能力及血清、肝脏和鸡蛋维生素A含量的影响

Effects of Vitamin A Level on Antioxidant Capacity in Serum,Vitamin A Contents in Serum,Liver and Egg of Laying Hens in Winter

本试验旨在研究冬季条件下维生素A水平对蛋鸡抗氧化能力及血清、肝脏和鸡蛋中维生素A含量的影响。选取525只43周龄健康海兰褐蛋鸡,随机分为5个组,每个组5个重复,每个重复21只鸡。对照组饲喂基础饲粮（维生素A水平为8 000IU/kg）,试验组饲粮维生素A水平分别为10 500、13 000、15 500和18 000IU/kg,其他维生素水平与对照组饲粮保持相同,试验期42d。结果表明：随维生素A水平的增加,血清总抗氧化能力和超氧化物歧化酶的活性呈先升后降趋势,血清丙二醛含量降低,肝脏中维生素A含量升高,且肝脏中维生素A含量与饲粮维生素A水平显著线性相关（P〈0.05）;第21和42天时,维生素A水平为8 000-15 500IU/kg时,随维生素A水平的增加,鸡蛋中的维生素A含量显著增加（P〈0.05）,而维生素A水平为15 500-18 000IU/kg时,随维生素A水平的增加,鸡蛋中维生素A含量反而显著降低（P〈0.05）。由此可知,在本试验条件下,饲粮维生素A水平为10 500-13 000IU/kg时,蛋鸡可以获得较强的血清抗氧化能力;维生素A水平为15 500IU/kg时,蛋鸡血清、肝脏和鸡蛋中可获得较高的维生素A沉积量。

This experiment was conducted to investigate the effects of vitamin A level on antioxidant function and vitamin A contents in serum, liver and egg of laying hens in winter. A total of 525 healthy Hy-line laying hens were randomly divided into 5 groups with 5 replicates per group and 21 hens per replicate. Hens in the control group were fed a basal diet （ contained 8 000 IU/kg vitamin A）, and the others in experimental groups were fed diets which contained 10 500, 13 000, 15 500 and 18 000 IU/kg vitamin A, respectively. All groups had the same level of other vitamins. The experiment lasted for 42 days. The results showed as follows ： the serum total antioxidant capacity （T-AOC） and superoxide dismutase （SOD） activity increased first and then decreased with the level of vitamin A increasing, however, the serum content of methane dicarboxylic aldehyde （MDA） was decreased. The content of vitamin A in liver was increased with the level of vitamin A increasing, moreover, the liver content of vitamin A was increased linearly （P〈0.05）. The content of vitamin A in eggs increased significantly with the level of vitamin A from 8 000 to 15 500 IU/kg （P〈0.05）, but the content of vitamin A in eggs decreased significantly with the level of vitamin A from 15 500 to 18 000 IU/kg on days 21 and 42 （P〈0.05）. In conclusion, dietary vitamin A level from 10 500 to 13 000 IU/kg can obtain the better antioxidant capacity, more contents of vitamin A can obtain in serum, liver and eggs with the level of vitamin A on 15 500 IU/kg.