心衰标志物可视化联合检测方法研究被引量：7

Rapid Visual Immunoassay of Multi-biomarker for Heart Failure Based on Magnetic Particles Images

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摘要基于夹心免疫原理和表面免疫吸附模型，采用Image Pro Plus软件对硅片表面磁颗粒分布图像进行定量化处理，建立了硅片表面心衰标志物可视化联合检测方法。研究表明，该方法从抗体包被到获得检测结果不超过1h，操作简单，在单张硅片上可实现心衰标志物NT_proBNP和cTnI的联合检测。NT—proBNP和cTnI的最低检出限分别为21．8pg／mL和0．034ng／mL，检测范围分别为21．8-40000pg／mL和0．034～50ng／mL。83例不同程度心血管疾病患者的血清样本的检测结果与商品化免疫分析仪检测结果一致，相关系数r〉0．98。因此，该快速免疫检测方法可以满足临床检测心衰标志物的需要。 Based on sandwich immunoassay, immuno-adsorption model and quantitative anal particles image on the surface of silicon chip, rapid immunoassay of multi-biomarkers for heart ysis of magnetic failure had been established. This method is simple and rapid compared to traditional ELISA. The assay time does not exceed 1 hour from antibody immobilization to final results obtained. In addition, it was easy to realize a joint measurement for NT-proBNP and cTnI on single silicon biochip. The limits of detection for NT-proBNP and cTnI were 21.8 pg/mL and 0. 034 ng/mL, and the detection ranges were 21.8 - 40000 pg/mL and 0. 034 - 50 ng/mL, respectively. The assay results for NT proBNP and cTnlwere compared using 83 serum samples from patients with different cardiovascular diseases with those on commercial analyzers and obtained a very good correlation （r〉0.98）. In conclusion, this rapid visual immunoassay is well suitable for the clinical detection of heart failure biomarkers.

作者霍卫松张磊高宇哲孟凡达高云华

机构地区中国科学院理化技术研究所光化学转换和功能材料重点实验室中国科学院大学

出处《影像科学与光化学》 CAS CSCD 北大核心 2015年第4期275-284,共10页 Imaging Science and Photochemistry

基金国家科技支撑计划(2013BAI03B)资助

关键词硅生物芯片可视化免疫检测方法心衰生物标志物 silicon biochip visualization immunoassay heart failure biomarker

分类号 R541.6 [医药卫生—心血管疾病] O652 [理学—分析化学]

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