HOXA10启动子区基因多态性与女性生殖能力的相关性研究

Investigation on the relationship of polymorphism of Hox110 promoter with the reproductive ability of females

目的探讨HOXA10启动子区基因多态性与女性生殖能力的关联性。方法选择我院2013年7月至2014年7月施行辅助生殖术(ART)的女性患者240例作为不孕组,其中三次施行ART未怀孕的患者归为ART失败组,同期产科自然怀孕且年龄偏大的女性250例作为自然怀孕组。检测两组患者HOXA10基因启动子区SNP位点rs3779456多态性,利用统计学软件SPSS19.0探讨其基因型及等位基因与女性生殖能力的关联性。结果三种基因型TT、CC、CT在不孕组和自然怀孕组间比较差异无统计学意义(P>0.05),在ART失败组和自然怀孕组间差异有统计学意义(P<0.05)。CC基因型和其余两种基因型的分布频率差异有统计学意义(P<0.05),且CC在不孕组和自然怀孕组分布频率的OR值为1.24。等位基因T和C的分布频率差异有统计学意义(P<0.05),且T在自然怀孕组和不孕组的分布频率OR值为1.15。结论基因型CC可能不利于自然怀孕,等位基因T可能与女性生殖能力呈正相关。

Objective To investigate the polymorphism of the HOXA10 promoter and the female reproductive ability. Methods A total of 240 female infertile patients undergoing assisted reproductive technology（ART） in our hospital from July 2013 to July 2014 were enrolled as infertility group, among which the patients was not pregnant after 3 times of ART were collected as the ART failure group. Two hundred and fifty aged females with normal reproductive ability were chosen as the control group. The polymorphism of rs3779456 located in the HOXA10 promoter was detected and the data were analyzed by SPSS19.0. Results There was no significant difference in the three genotypes（TT, CC, CT） between the infertility group and the control group（P〉0.05）, while there was statistically significant difference between ART failure group and the control group（P〈0.05）. The frequency of CC and the other two genotypes was significantly different（P〈0.05）, the OR value of CC in the infertility group and the control group was1.24. The allele frequency of T and C between infertility group and the control group were significantly different with an OR value of 1.15. Conclusion The genotype CC may be a dangerous factor for natural pregnancy, while the allele T is beneficial for the reproductive ability.