α-倒捻子素对小鼠视网膜光损伤的保护作用

Protective effect of α- mangostin on retinal light damage in mice

目的：探讨α-倒捻子素对小鼠视网膜光损伤的保护作用。方法：取健康6-8wk Balb/c小鼠30只,随机分成正常对照组、光照组、α-倒捻子素组,每组10只。α-倒捻子素组小鼠每天接受α-倒捻子素30mg/（kg.d）灌胃7d,于灌胃给药第5d进行光损伤造模。对光照组和α-倒捻子素组小鼠采用5000±200lx白色LED光连续照射1h。模型完成后72h记录各组小鼠闪光视网膜电图;应用光镜观察各组小鼠视网膜形态学变化;剥离视网膜,检测各组小鼠视网膜组织匀浆中丙二醛（MDA）含量。结果：闪光视网膜电图（F-ERG）显示α-倒捻子素组视网膜功能损伤较光照组明显减轻（P〈0.05）。光镜下,α-倒捻子素组视网膜结构损伤相较于光照组明显减轻（P〈0.05）。相对于光照组,α-倒捻子素组视网膜组织MDA含量明显减少（P〈0.05）。结论：α-倒捻子素抑制光损伤引起的视网膜脂质过氧化,对小鼠视网膜光损伤起到保护作用。

AIM ： To discuss the protective effect of α-mangostin on retinal light dam age in mice.METHODS： Totally 30 Balb /c mice,aged 6 - 8wk,were randomly divided into the control group,light- exposure group and α-mangostin group. Every group contained 10 mice. Mice of α-mangostin group were treated with alphamangostin at the dose of 30 mg /（ kg ·d） body weight by intragastric administration daily for 7d,and then exposed to white light at the 5^th d. The light- exposure group and α-mangostin group were exposed to 5000 ± 200 lx white light-emmiting diodes（ LEDs） for continuously 1h to establish the mice model of retinal light damage. Flash-electroretinograme was recorded 72 h after light exposure.The changes in retinal morphology of mice were observed by light microscopy. Retinas were extracted to detect the m alondialdhyde（ M DA） content change of the retinal hom ogenate.RESULTS： Flash-electroretinogram（ F-ERG） showed that retinal dysfunction was less severe in α- mangostin group than in light- exposure group（ P〈0. 05）. Light microscopy test showed that retina structural dam age was less severe in α-mangostin group than in light-exposure group（ P0. 05）. The level of M DA in retinal tissue of α-mangostin group was significantly low er when com pared with light-exposure group（ P0. 05）.CONCLUSION： α-mangostin inhibits lipid peroxidation induced by light damage and protect retina against light dam age.