基于β-香树脂醇合成酶基因SNP的甘草道地性机制研究

Mechanism of genuineness of Glycyrrhiza uralensis based on SNP of β-Amyrin synthase gene

本文采用PCR-SSCP和测序组合技术检测了6个产地甘草的β-香树脂醇合成酶(β-AS)基因的SNP,并进行了SNP和产地的相关性分析,探索甘草道地性形成的分子机制。结果显示,甘草β-AS基因第一外显子存在1处SNP,可将甘草划分为94A型、94G型和94A/G型3种基因型,其中94A型在道地产区所占比例要远远高于非道地产区,达到极显著差异(P<0.001)。本文研究证明,甘草β-AS基因94位点的SNP可能是导致甘草道地性形成的机制之一。

β-Amyrin synthase（β-AS） genes of Glycyrrhiza uralensis from 6 different regions were analyzed by PCR-SSCP and sequenced, then the correlationship between β-AS SNP and regions of Glycyrrhiza uralensis were determined. According to the 1 coding single nucleotide polymorphism on the first exon of β-AS gene at 94 bp site, Glycyrrhiza uralensis could be divided into 3 genotypes. In these genotypes, the percentage of 94 A type in genuine regions was much higher, and it had significant differences with the percentage in non-genuine regions（P〈 0.001）. The results of the experiment proved that different β-AS genotypes at 94 bp site from different regions may be one of the important reasons to result in the genuineness of Glycyrrhiza uralensis.