两种高脂饲料分段喂养对大鼠体重和血脂的影响

Influence of Sequential Feeding Two Kinds of High-fat Diets on Body Weight and Blood Lipids of Rats

目的探索两种高脂饲料分段喂养对大鼠体重和血脂的影响.方法选取30只健康雄性SD大鼠,随机分为2组：对照组和实验组,每组15只.对照组大鼠以基础饲料喂养,实验组大鼠在腹腔注射VD3基础上,分阶段先后给予A和B 2种不同的高脂饲料喂养（1-7周给予A种高脂饲料,8-16周给予B种高脂饲料）.每周记录各组大鼠体重变化,每2周测定各组大鼠血脂（TC、TG、LDL-C、HDL-C）水平变化.结果对照组大鼠体重持续增加,实验组大鼠前2周体重增加,第3-7周开始持续降低,第8周更换B种高脂饲料后,体重又开始持续增加.两组体重除实验前和第16周差异无统计学意义外,其余时间点差异均有统计学意义（P〈0.05）.对照组大鼠血脂水平在实验期间无明显变化.实验组大鼠血清TC、HDL-C和LDL-C水平先升后降,TC在第6周达到峰值,HDL-C和LDL-C在第4周达到峰值.实验组大鼠血清TG水平在前6周无明显变化,到第8周更换B种高脂饲料后开始缓慢上升,至第16周时,明显高于对照组（P〈0.05）.结论 2种高脂饲料分段喂养可加速大鼠动脉粥样硬化模型的建立.

Objective To explore influence of sequential feeding two kinds of high- fat diets on the body weight and blood lipids of rats.Methods 30 SD rats（male） were randomly divided into two groups： control group and study group（n=15 in each group）. Rats in the study group were given sequential feeding two kinds of high- fat diets（high- fat diet A for 1- 7 week and high- fat diet B for 8- 16 week） on the basis of intraperitoneal injection of VD3（750 000 U/kg）. Rats in the control group were only given the the basal diet. The body weight of rats were recorded once every week and the blood lipids（TC,TG,LDL- C and HDL- C） were tested every two weeks.Results The body weights of rats in the control group increased continuously during this study. The body weights of rats in the study group increased in the first 2 weeks,decreased continuously from the 3rd week to the 7th week,then increased continuously from the the 8th week after changing high- fat diet A with B. There were statistically significant differences in the body weights between the two groups at different time points except before study and the16 th week after study（P〈0.05）. The blood lipids levels of rats in the control group were stable during the study. In the control group, the srum levels of TC, HDL- C and LDL- C of rats increased firstly and then decreased, and reached the peak values on the 6th and 4th week, respectively; the srum levels of TG had no significant change in the first 6 weeks,and gradually increased from the 8th week after changing high- fat diet A with B, and were significantly higher than those in the control group（P〈0.05）.Conclusion Sequential feeding two kinds of high- fat diets may accelerate the establishment of rat model of atherosclerosis.