罗格列酮调控光老化成纤维细胞周期阻滞的研究

Regulation of rosiglitazone in the cell cycle arrest of photoaging fibroblasts

目的 探讨罗格列酮对光老化成纤维细胞的周期及相关蛋白的影响.方法 采用cck-8法检测罗格列酮对成纤维细胞增殖的影响.罗格列酮预处理成纤维细胞48 h,采用UVB 120 mJ/cm2反复照射4次,照射间隔时间12h,诱导产生细胞光老化模型,继续培养48 h,流式细胞技术检测成纤维细胞周期,Western Blot检测周期相关蛋白p53和p21.结果 低剂量罗格列酮对成纤维细胞的活性无影响.40 μM罗格列酮预处理的光老化成纤维细胞与单纯UVB照射组相比,S期的比例下降了15.9％.与对照组相比,40μM罗格列酮预处理组中成纤维细胞周期相关蛋白p53和p21的表达分别下降了35.7％和27.7％.结论 罗格列酮可降低光老化成纤维细胞周期的相关蛋白p53和p21的表达,缓解UVB引起的光老化成纤维细胞的S期阻滞,可能对光老化成纤维细胞具有某种保护作用.

Objective To investigate the influence of rosiglitazone in cell cycle and associated proteins. Methods The influence of rosiglitazone on fibroblasts proliferation was measured by cck-8 assay. After rosiglitazone pretreatment for 48 h, fibroblasts were irradiated by a series of 4 UVB exposures at the dose of 120 mJ/cm2 with an interval time of 12 h. The influence of rosiglitazone on fibroblasts cycle was analyzed by flow cytometry and the expressions of cell cycle regulatory proteins p53 and p21 were evaluated by Western Blot 48 h after the last exposure. Results No modification in the proliferative activity of MDFs was observed at any dose tested up to 80 μM after 48 h （ P 〈0.01 ）. The cell cycle arrest in S phase of fibroblasts pretreated with rosiglitazone was 15.9 % less than the UVB group. The expressions of cell cycle regulatory proteins p53 and p21 were decreased by 35.7% and 27.7% respectively compared with UVB group. Conclusion Rosislitazone could decreased the expressions of p53 and p21, cell cycle regulatory proteins of photoaging fibroblasts, relieve the cell cycle arrest of photoaging fibroblasts caused by UVB, so its application may have some protective effect on photoaging fibroblasts.