离子色谱-质谱联用法测定4-甲基咪唑的含量

Determination of 4-Methylimidazole Content via Ion Chromatography-Mass Spectrometry

为了简单、方便地精确定量和定性样品中的4-甲基咪唑,建立了一种采用阳离子交换色谱-质谱联用（IC-MS）技术准确检测4-甲基咪唑含量的新方法,该方法的检出限为0.25μg/L,线性范围为2.5~200μg/L,线性相关系数为0.999 7,相对标准偏差为2.32%~4.25%,加标回收率为92.7%~96.3%.使用该方法对焦糖色素、可乐等样品进行检测,结果表明,该方法具有分离度高、灵敏度高、定量准确、精密度高、检出限低的特点.

In order to quantitatively and qualitatively determine the content of 4-methylimidazole in samples with simplicity,convenience and accuracy,a new method using cation exchange chromatography-mass spectrometry was developed. This method,with a detection limit of 0. 25μg / L,a linear range varying from 2. 5 to 200μg / L,a linear correlation coefficient of 0. 999 7,a relative standard deviation of 2. 32% ~ 4. 25% and a recovery varying from92. 7% to 96. 3%,was then used to test the 4-methylimidazole in caramel pigment,cola and some other samples.The results show that the proposed method is of high separation degree,good sensitivity,accurate quantification,high precision and low detection limit.