微槽透光板式光生物制氢反应器底物操作参数优化

Substrate Operating Parameters Optimization on Hydrogen Production within Groove-type Flat-panel Photobioreactor

从连续流产氢的目标出发,采用在高导光性载体表面刻制微槽的方法,构造了具有高比表面积的微槽透光板式光生物制氢反应器,完成了光合细菌的快速成膜并实现细胞固定化。用Box-Behnken的响应曲面分析方法,对影响微槽透光板式光生物制氢反应器连续流产氢性能的相关底物操作参数进行了3因素3水平的实验。结果表明,对反应器产氢速率的单因素影响显著顺序依次为水力停留时间、初始pH值和初始底物浓度。对反应器产氢速率具有显著交互作用的影响因素是初始底物浓度和初始pH值。当初始底物浓度、水力停留时间和初始pH值分别为54.3 mmol/L、21.5 h和7.1时,微槽透光板式光生物制氢反应器的产氢速率达到最大值2.242 mmol/(L·h)。

To realize photo-biological hydrogen production under continuous flow mode,a groove-type flat-panel photobioreactor（ GFBR） with high specific surface area was developed by carving groove on the surface of high-light transparent medium. With photosynthetic bacteria attached on the groove-type surface and the generated mature biofilm,cell-immobilization was finished within GFBR. To improve the performance of hydrogen production in GFBR, operating parameters related to substrate were comprehensively investigated using response surface methodology of Box-Behnken with three factors and three levels. The results showed that the significant degree of single factor on hydrogen production rate of GFBR was followed as hydraulic retention time,initial pH value and initial substrate concentration.While,interaction effect between initial pH value and initial substrate concentration on hydrogen production rate of GFBR was also significant. The optimum hydrogen production rate of 2. 242 mmol /（ L·h）was attained with operating parameters of initial substrate concentration of 54. 3 mmol / L,hydraulic retention time of 21. 5 h and initial pH value of 7. 1.