HPLC法测定白背叶中3个黄酮碳苷类成分的含量

HPLC method for simultaneous determination of three flavonoids in Mallotus apelta

目的：建立高效液相色谱法同时测定白背叶药材中葫芦巴苷Ⅱ、异夏佛托苷、夏佛托苷3个黄酮碳苷类成分含量。方法：采用Agilent Eclipse XDB-C18色谱柱（4.6 mm×250 mm,5μm）,柱温25℃,流动相为甲醇-0.1%乙酸水溶液,梯度洗脱,流速1.0 m L·min-1,检测波长336 nm。结果：葫芦巴苷Ⅱ、异夏佛托苷、夏佛托苷3个成分的色谱峰均达到基线分离,其进样量分别在0.026 6∽5.32、0.024 4∽4.88、0.024 2∽4.84μg范围内线性关系良好（r=0.999 0）,平均加样回收率（n=6）分别为97.3%、100.1%、103.0%;不同批号的白背叶药材葫芦巴苷Ⅱ、异夏佛托苷、夏佛托的含量分别为0.73∽7.48、1.25∽7.58、1.03∽5.89 mg·g-1。结论：本文所建立的含量测定方法经方法验证适于白背叶药材的质量检测。

Objective ：To establish an HPLC method for simultaneous determination of three flavone glycosides （vi- cenin II , isoschaftoside and schaftoside） in Mallotus apeha. Methods： HPLC was performed on an Agilent Eclipse XDB-C18 analytical column （4. 6 mm ×250mm,5 μm） at 25 ℃ with methanol and 0. 1% acetic acid as the mobile phase by gradient elution,the flow rate was 1.0 mL · min-1 and the detection wavelength was 336 nm. Results：The baseline separation of these three components was achieved; the linear ranges of vicenin Ⅱ , isoschaftoside and schaftoside were 0. 026 6-5.32,0. 024 4-4. 88, and 0. 024 2-4. 84 Ixg, respectively （r = 0. 999 0） ; the average recoveries （ n = 6 ） were 97.26% , 100. 1, and 103.0% , respectively. The content ranges of vicenin 11 , isoschaftoside and schaftoside were 0. 73-7.48,1.25-7.58 and 1.03-5.89 mg·g-1for MaUotus apelta from different batch num- ber. Conclusion：The established method of determination can be used for quality control of Mallotus apeha.