肉豆蔻薄层色谱用对照提取物的制备与应用研究

Study on the preparation and application of nutmeg TLC reference extract

目的:研究制备肉豆蔻薄层色谱用对照提取物,探讨对照提取物替代对照药材在肉豆蔻药材及其制剂质量控制中的应用。方法:通过药材筛选、制备工艺摸索、稳定性影响因素考察等,研制肉豆蔻薄层用对照提取物,并应用于肉豆蔻药材、二十五味松石丸、四神丸等中国药典2010年版一部药品标准中的薄层色谱鉴别项。结果:肉豆蔻薄层用对照提取物与肉豆蔻对照药材薄层色谱效果一致,等量同质。结论:研制了定性用的肉豆蔻薄层色谱用对照提取物标准物质,并用于肉豆蔻药材及肉豆蔻类制剂的质量控制分析,为中药对照提取物的进一步发展提供研究基础。

Objective：To prepare nutmeg TLC reference extract, and explore its application in the quality control of Myristicae Semen and nutmeg preparations as a reference drug. Methods：Nutmeg TLC reference extract was pre- pared by screening crude drug, opting processing route and investigating stability influencing factors etc. The appli- cation to TLC identification items of Myristicae Semen, Ershiwuwei Songshi Wan and Sishen Wan was performed ac- cording to the Chinese Pharmacopoeia 2010 edition I. Results：Nutmeg TLC reference extract can play the same role as Myristicae Semen reference drug with the same quantity and quality. Conclusion：Nutmeg TLC reference ex- tract for TLC identification was prepared, which can be used for quality control analysis of Myristieae Semen and Nutmeg preparations. The research provided a foundation for further development of TCM reference extracts.