PTEN和Ki-67在结肠癌中的表达及其意义

Expression and clinical significance of PTEN and Ki-67 in colon Cancer

目的研究PTEN蛋白和Ki-67蛋白在结肠癌组织中的表达,并探讨它们的相关性及临床意义。方法采用免疫组化SP法检测56例结肠癌组织和20例结肠腺瘤组织及正常结肠组织中PTEN蛋白和Ki-67蛋白的表达情况。结果 PTEN蛋白在结肠癌、结肠腺瘤、正常结肠组织中的阳性表达率分别为26.79%(15/56)、55%(11/20)、90%(18/20);Ki-67蛋白的阳性表达率分别为85.71%(48/56)、50%(10/20)、15%(3/20);组间进行比较,差异具有统计学意义(P<0.05)。PTEN蛋白和Ki-67蛋白的异常表达与肿瘤的分化程度、有无淋巴结转移、Dukes分期有关(P<0.05);且二者呈负相关。结论联合PTEN蛋白和Ki-67蛋白的表达对判断结肠癌临床分期、预测转移趋势、预后评估具有重要的临床意义。

Objective To explore the expression and clinical significance of PTEN and Ki-67 proteins in colon cancer,and to investigate the correlation and clinical significance of PTEN and Ki-67 proteins expression.Methods Expression of PTEN and Ki-67 in 56 cases of colon cancer and 20 cases of colonic adenoma and normal colon tissue was determined by immunohistochemical staining,and relationship between PTEN and Ki-67 expression and pathological features in colon cancer. Results PTEN protein in colorectal cancer, colonic adenoma and normal colon tissue in the positive expression rate was 26. 79%（ 15 /56）,55%（ 11 /20）,90%（ 18 /20）; Ki- 67 protein positive expression rate was 85. 71%（ 48 /56）,50%（ 10 /20）,15%（ 3 /20）;Between the two groups were compared,the difference was statistically significant（ P〈0. 05）,PTEN protein and Ki-67 protein abnormal expression is closely related to tumor differentiation degree,with or without lymph node metastasis,Dukes staging（ P〈0. 05）; and a negative correlation between them. Conclusions Joint detection PTEN protein and Ki-67 protein for judging the trend of colon cancer clinical stage,predicting metastasis and prognosis assessment has important clinical significance.