胆管癌组织中Wnt2b蛋白表达及其临床意义

EXPRESSION OF Wnt2b IN CHOLANGIOCARCINOMA AND ITS CLINICAL SIGNIFICANCE

目的观察Wnt2b蛋白在胆管癌组织的表达,并探讨其在胆管癌发生发展及浸润转移过程中的临床意义。方法采用免疫组化染色方法检测60例胆管癌及30例胆管正常组织中Wnt2b蛋白的表达情况,并分析其与胆管癌临床特征的关系。结果 Wnt2b在胆管癌组织的阳性率(90.00%)明显高于正常胆管组织(3.33%),差异有显著性(χ2=38.13,P<0.01)。Wnt2b表达与胆管癌的浸润深度和淋巴结转移程度相关(Z=-3.171、-3.316,P<0.01),而与病人的年龄、性别、胆管癌发生部位及胆管癌的分化程度无关(Z=-0.048^-1.020,P>0.05)。结论 Wnt2b蛋白可能作为评估胆管癌临床分期与淋巴结转移的一种有价值的生物学标记。

Objective To observe the expression of Wnt2 bprotein and investigate its clinical significance in the development,progression,invasion and metastasis of cholangiocarcinoma（CLC）. Methods Employing immunohistochemical method,the expression of Wnt2 bprotein in tissues of 60 cases of CLC and 30 normal bile duct tissues was detected,and its relationship with clinical characteristics of this malignancy was analyzed. Results The positive rate of Wnt2 bwas significantly higher in CLC than that in normal bile duct tissue（90.00% vs.3.33%,χ^2=38.13,P〈0.01）.The expression of Wnt2 bwas positively correlated with depth of tumor invasion and lymph node metastasis（Z=-3.171,-3.316;P〈0.01）,but it was not related to patient＇s age,gender,tumor location and cell differentiation（Z=-0.048--1.020,P〉0.05）. Conclusion Wnt2 bis probably to be used as a useful biological marker for assessing clinical stage and lymph node metastasis of cholangiocarcinoma.