精浆弹性硬蛋白酶与精子DNA完整性及精液参数的影响分析

Analysis of seminal plasma elastase with sperm DNA integrity and semen quality in infertile men

目的探讨男性不育患者精浆弹性硬蛋白酶水平与精子DNA完整性及精液质量之间的关系。方法选择148例男性不育患者根据精浆弹性硬蛋白酶的检测结果将所有患者分为A、B、C3组。A组为精浆弹性硬蛋白酶含量〈290ng／mL；B组为精浆弹性硬蛋白酶含量290～1000ng／mL；C组为精浆弹性硬蛋白酶含量〉1000ng／mL。用酶联免疫吸附试验（ELISA）检测精浆中弹性硬蛋白酶，精子DNA完整性检测用精子染色质扩散法（SCD），精液参数用计算机辅助精子分析系统检测。结果A组与C组相比精子存活率与前向运动精子百分率（PR）均升高、精子DNA碎片指数（DFI）明显降低，差异均具有统计学意义（P〈0．05）。B组的上述指标与A组相比，差异均无统计学意义（P〉0．05）。结论精浆弹性硬蛋白酶与精子DNA完整性及精液质量密切相关，生殖道感染是影响男性精液质量的一个重要原因。

Objectives To study the relationship of the seminal plasma elastase （ SPE ） level with sperm DNA integrity and semen quality in infertile men. Methods Based on the results of seminal plasma elastase tests, 148 infertile men were divided into group A（SPE： 〈290 ng/mL）, group B（ SPE：290 - 1000ng/mL） ,and groupC（ SPE ： 〉 1000ng/mL）. The level of SPE was detected by ELISA,integrity of sperm DNA weew used sperm ehromation dispersion test to measure, and analyses were also performed on the semen parameters by CASA. Results Sperm livability and the rate of progressively motile sperm（PR） in group A were all higher than that in group C,and the DFI was apparently depressed （ P 〈 0.05 ）. But there was no significant difference in the indexes above between group B and group A（ P 〉 0.05）. Conclusions The results suggested that seminal plasma elastase was closely assoeiat- ed with sperm DNA integrity and semen quality, meanwhile the reproductive tract infection is an important factor af- feeting the male semen quality.