钩枝藤提取物的生物活性

Biological Activity of the Extracts from Ancistrocladus tectorius

通过观察钩枝藤乙醇提取物治疗感染了伯氏疟原虫小鼠的感染率和存活率判断钩枝藤乙醇提取物的抗疟活性;采用MTT法测定钩枝藤乙醇提取物体外细胞毒活性;采用滤纸片琼脂扩散法测定钩枝藤乙醇提取物抗菌活性;采用Ellman比色法测试钩枝藤乙醇提取物抗乙酰胆碱酯酶活性。结果表明,该提取物能较好地抑制伯氏疟原虫的生长,改善小鼠的存活状况;对人白血病细胞K562、胃癌细胞SGC-7721、肝癌细胞BEL-7402均具有较好的抑制作用;对人体病原菌和农业病原菌表现出较强的抑菌活性;对乙酰胆碱酯酶表现出一定的抑制作用,但不如阳性对照他克林。

Ancistrocladus tectorius was extracted with ethyl alcohol （EtOH）, and the active constituents of the EtOH and their biological activities against malaria were determined. Mice infected with Plasmodium berghei were treated with the EtOH extract, and its cytotoxic activity was assayed by the MTI＂ method. The antimicrobial activi- ties of the EtOH extract were tested by the paper disk diffusion method, and its acetylcholinesterase inhibitory ac- tivity was detected by using the Ellman＇ s colorimetric method. The results indicated that the EtOH extract of A. tectorius inhibited the growth of malaria parasites and improved the survival rate of the mice infected with P. berghei. It also possessed inhibitory activity against K562, SGC-7721 and BEL-7402 cell lines, as well as human and agricultural pathogens. Moreover, it showed lower inhibitory activity against acetylcholinesterase than Tacrine.