国产沉香TLC的指纹图谱分析

TLC Fingerprint of Agarwood of Aquilaria sinensis

通过2个展开系统和3种检视方法,建立10批国产沉香的薄层色谱(TLC)指纹图谱。结果表明,该方法精密度、稳定性和重复性良好,符合指纹图谱技术要求。10批样品中检测到17个共有斑点,初步鉴定了其中6个,包括3个色酮类成分,2个倍半萜类成分和1个谷甾醇。

The TLC fingerprints of ether extract of ten agarwood samples from Aquilaria sinensis were established by using the thin-layer chromatography （TLC） with two developing solvents and three observation methods, UV 254 nm, UV 365 nm and visualization reagent 5% vanillin sulfuric acid/ethanol. The results show that the TLC fingerprinting method was precise, repeatable and stable, meeting the fingerprint qualification. The ten batches of agarwood samples showed 17 common fluorescent spots, of which seven spots were characterized, including 4 chronmones, 2 sesquiterpenes and a sitosterol. The TLC fingerprinting could quickly, intuitively and reliably re- flect the whole characteristics, distinguish agarwood from Aquilaria sinensis expediently, and it was a quick and effective method for true and false identification of agarwood.