摘要
研究濒危藏药肉叶金腰内生真菌Cladosporium sp.J6次级代谢产物。采用硅胶、LH-20凝胶、C-18反相硅胶及HPLC等柱色谱技术对Cladosporium sp.J6的次级代谢产物进行研究;采用波谱分析及与文献比对等方法进行化合物结构鉴定;结果从肉叶金腰内生真菌Cladosporium sp.J6的发酵提取物中分离得到5个化合物,分别鉴定为alternariol(1)、alternariol 5-O-methyl ether(2)、β-咔啉(3)、尿嘧啶(4)和尿嘧啶核苷(5)。关于金腰属植物内生真菌次级代谢产物研究是首次报道。
To study the secondary metabolites of endophytic Cladosporium sp. J6 from endangered Chrysosplenium carnosum from Tibet. The chemical constituents were isolated and purified by silica gel,Sephadex LH- 20,ODS- 18 column chromatography,and HPLC; their structures were identified by NMR and MS analysis,or comparison with literatures. Five compounds were isolated from the fermentation products of Cladosporium sp. J6; their structures were identified as alternariol（ 1）,alternariol 5-Omethyl ether（2）,β-carboline（3）,uracil（4）,and uridine（5）. This report was about secondary metabolites of endophytic fungus from Chrysosplenium Tourn. ex L. for the first time.
出处
《中山大学学报（自然科学版）》
CAS
CSCD
北大核心
2015年第4期84-86,共3页
Acta Scientiarum Naturalium Universitatis Sunyatseni
基金
西藏自治区科技计划重资助项目"毛瓣绿绒蒿等濒危藏药材内生菌次级代谢及其与宿主互惠共存关系研究"(2011)
国家自然科学基金地方科学基金资助项目(21262033)
关键词
肉叶金腰
内生真菌
分离鉴定
化学成份
Chrysosplenium carnosum
endophytic fungus
isolation
secondary metabolites
