100种常用藏药抗肝癌的体外活性筛选

In vitro Anti-hepatoma Screening of 100 Commonly Used Tibetan Medicines

为从藏药中发现新的抗癌药物,从青海互助县收集了100种常用藏药,采用快速溶剂萃取法制备出了各种藏药的环己烷、乙酸乙酯和甲醇提取物.选用2种肝癌细胞株Hep G2和SMMC-7721,以MTT法测试了100种藏药的抗肝癌活性,选用人肝细胞株LO-2评价了藏药的体外毒性.结果表明:15种藏药材显示出抗肝癌活性,IC50值均小于150μg/m L,其中藜、天名精、三脉紫菀3种藏药的乙酸乙酯提取物显示出显著的抗肝癌活性,其IC50值均小于50μg/m L,而对正常肝细胞LO-2未显示出明显的毒性.该研究为进一步从藏药中发现新的抗癌药物提供了思路.

To discover new anticancer drugs from Tibetan medicines,the cyclohexane,ethyl acetate and methanol extracts from 100 commonly used Tibetan medicines collected from Huzhu County, Qinghai Province were prepared by an accelerated solvent extraction method. The MTT method was used to test the anti-hepatoma activity of the 100 Tibetan medicines against two hepatocellular carcinoma cell lines Hep G2 and SMMC-7721,and the preliminary cell toxicity was investigated using a human liver cell line LO-2. The results indicated that 15 of these Tibetan medicines exhibited antihepatoma activities against Hep G2 and SMMC-7721 with IC50 values less than 150 μg / m L. Among these,the ethyl acetate extracts of Delphinium caeruleum,Dracocephalum tanguticum and Ligularia przewalskii showed significant antitumor activity with IC50 values less than 50 μg / m L,yet they didn＇t show obvious toxicity against LO-2 cells. This research presented a new way to discover new anti-hepatoma drugs from Tibetan medicines.