UPLC法测定止嗽化痰胶囊中吗啡、可待因和罂粟碱的含量

Determination of Morphine,Codeine and Papaverine in Zhisou Huatan Capsules by UPLC

目的:建立止嗽化痰胶囊中吗啡、可待因和罂粟碱含量的UPLC测定方法。方法:采用ACQUITY UPLC HSS C18柱(2.1 mm×100 mm,1.8μm),以乙腈(A)-0.2%三氟乙酸(B)梯度洗脱;流量为0.4 mL·min^(-1),柱温35℃,检测波长为237nm。结果:吗啡、可待因和罂粟碱的线性范围分别为0.005~0.131μg(r=0.999 4)、0.002~0.061μg(r=1.000)、0.001~0.025(r=1.000),平均回收率(n=6)分别为98.8%、95.2%、101.1%,其RSD分别为2.2%、2.8%、3.3%。结论:本法可靠、准确,可用于该制剂的质量控制。

Objective：To establish an UPLC method for determining Morphine, Codeine and Papaverine in Zhisou Huatan Capsules.Methods：A ACQUITY UPLC HSS C18 column （2.1 mm ×100 mm, 1.8 μm） was adopted with acetonitrile as mobile phase A, 0.2%trifluoroacetic acid as mobile phase B, gradient elution.The flow rate was 0.4 mL· min^-1 , column temperature was 35 ℃and detection wavelength was 237 nm.Results：The linear range of Morphine, Codeine and Papaverine was 0.005 -0.131 μg （r=0.999 4）, 0.002-0.061 μg（r=1.000）and 0.001-0.025（r=1.000）,respectively.The average recovery rate of Morphine, Codeine and Papaverine was 98.8%,95.2%and 101.1%（ n=6） with RSD of 2.2%, 2.8%and 3.3%, respectively.Conclusion：The method is reliable,accurate and can be used for the quality control of the preparation.