甲型流感病毒分支状M2e多肽疫苗不同途径免疫效果的研究

Effects of branched M2e polypeptides influenza A vaccine immunized through different routes

目的 比较甲型流感病毒分支状多肽疫苗最佳免疫途径.方法 通过化学方法合成（M2e） 4-tuftsin,与Al（OH）3佐剂混合,肌注途径免疫小鼠;与JY佐剂混合,滴鼻免疫小鼠,观察两种途径的免疫效果.结果 肌内注射免疫途径诱导产生抗体的能力高于滴鼻免疫,（M2e） 4-tuftsin滴鼻免疫诱导细胞免疫的能力最强,（M2e）4-tuftsin通过肌肉注射和滴鼻免疫途径均对小鼠有一定的保护效果,但肌注免疫效果最好.结论 分支状M2e多肽肌内注射比滴鼻免疫保护效果好。

Objective To compare the immune effects induced with universal influenza A vaccine based on branched M2e polypeptides by two immunization routes in mice.Methods The M2e monomer and （M2e）4-tuftsin were synthesized using chemical method.One group of mice were immunized intramuscularly （i.m.） with 10 μg of （M2e）4-tuftsin plus aluminum adjuvant.And the other group of mice were intranasally immunized with the same amount of polypeptides plus JY adjuvant.Then the immune effects of different immune pathways were observed.Results Intramuscular route induced higher level of M2 specific antibody than intranasal route.Intramuscular injection of （M2e）4-tuftsin was the most effective in stimulating T cell responses.Both intramuscular and intranasal vaccinations （M2e） 4-tuftsin had protective effect,but intramuscular vaccination could provide the most efficient protective immunity.Conclusion The data indicated that the intramuscular vaccination could be the better way for immunization than intranasal vaccination.