南京2011-2013年婴幼儿腹泻轮状病毒分子流行病学研究

The monitoring on molecular epidemiology of rotavirus diarrhea of children in Nanjing city during 2011-2013

目的 研究2011年7月至2013年6月南京地区婴幼儿轮状病毒分子流行病学特点.方法 收集2011年7月至2013年6月南京市儿童医院腹泻门诊5岁以下腹泻患儿粪便标本807例,采用酶联免疫吸附试验进行轮状病毒检测,使用巢式PCR法结合测序对轮状病毒进行基因分型.结果 807份腹泻标本中,轮状病毒阳性251份,总检出率31.1％;感染对象主要以2岁以下儿童为主,腹泻流行高峰为10月至次年1月.其中2011-2012年度,2012-2013年度轮状病毒检出率分别为30％和32.19％.对251份轮状病毒阳性标本G/P分型结果显示,G基因型主要以G9、G3为主,其中G9型阳性率为53.39％,G3型阳性率为16.33％,P基因型主要以P[8]型为主,阳性率为86.1％.2011-2012年度轮状病毒流行毒株以G9P[8]（28.3％）、G3P[8] （25％）、G1P[8] （17.5％）及G2P[4]（15％）四种为主,2012-2013年度以G9P[8]（72.52％）为主.结论 轮状病毒在南京地区婴幼儿病毒性腹泻中占重要地位,RV G9型取代G3型,逐渐成为本地区主要流行毒株。

Objective To investigate the molecular epidemiology of rotavirus infected in children with viral diarrhea from July 2011 to June 2013 in Nanjing.Method 807 stool specimens of outpatient children with diarrhea in Nanjing Children＆#39;s Hospital were collected from July 2011 to June 2013.Rotavirus were tested by enzyme-linked immunosorbent assay and the positive samples further were test with nested RT-PCR methods.The sequences of gene fragments are analyzed.Result In the 807 specimens,31.1% （251） specimens were rotavirus-positive.Patients infected with RV were mainly under 2 years old and the rotavirus diarrhea predominated in spring and winter months.The detection ratios of rotavirus were 30% and 32.19% in 2011-2012 and 2012-2013 respectively.WithinVP7 serotype of rotavirus-positive specimens,G9 and G3 were the major G type,accounted for 53.39% and 16.33% respectively.P [8] was the mainly genotype in VP4 serotype,which was accounted for 86.1%.According to G/P type,G9P[8] （28.3%）,G3P[8]（25%）,G1P[8]（17.5%）andG2P[4]（15%） were the main kinds of Rotavirus strains in 2011-2012 as well as G9P [8] （72.52%） in 2012-2013.Conclusion RV occupied an important status in children with viral diarrhea in young children in Nanjing.Rotavirus G9 genotype were more prevalence than G3 genotype,becoming a major strains in Nanjing.