不同尺寸金纳米颗粒在小鼠胚胎干细胞胚状体中富集和分布的研究

Size-dependent distribution and accumulation of gold nanoparticles in mouse embryonic stem cells

随着金纳米材料相关生物医学应用的不断深入,其生物学效应研究备受关注。金纳米材料对胚胎发育的影响,是其生物学效应的重要研究内容。本研究中制备了2 nm、15 nm、50 nm三种具有统一表面修饰的金纳米颗粒,利用透射电子显微镜和电感耦合等离子体质谱仪等多种方法,定性、定量地观察不同尺寸的金纳米颗粒在小鼠胚胎干细胞胚状体中的分布和富集情况。结果显示,2 nm、15 nm、50 nm三种金颗粒的富集情况分别为(38.02±2.01)ng/105cells、(34.27±3.42)ng/105cells以及(1 435.97±65.33)ng/105cells,而且在细胞内的分布区域不尽相同。说明金纳米颗粒在小鼠胚胎干细胞胚状体中的累积量与其尺寸相关,不同尺寸的金颗粒显示出不同的胞内分布。这一发现将有助于进一步阐明金纳米颗粒的尺寸效应对胚胎干细胞分化、发育过程的影响。

Due to the wide application of gold nanomaterials to the biomedical field, the biological effect has attracted more and more attention. The study of gold nanoparticles’ influence on embryonic development is an important aspect of the biological effects. We synthesized a series of sizes （2, 15, 50 nm） of gold nanoparticles with the same surface modification to illustrate the size effects on accumulation and distribution of gold nanoparticles in embryoid bodies. We found that gold nanoparticles-treated embryoid bodies exhibited gold element accumulation of （38.02 ±2.01）ng/10^5, （34.27 ±3.42）ng/10^5, and （1 435.97 ±65.33）ng/10^5 cells after incubation with 2, 15, and 50 nm gold nanoparticles. Size-dependent intracellular distribution was then observed in this study, which provided an important basis for the follow-up study to explain the effect of gold nanoparticles on the differentiation of embryonic stem cells.