Klotho基因G-395A多态性与慢性肾脏病患者动脉硬化的关系被引量：2

Association of G-395A Polymorphism of Human Klotho Gene with Arteriosclerosis in Patients of Chronic Kidney Disease

下载PDF

导出

摘要目的:研究慢性肾脏病非透析患者Klotho基因启动子区域G-395A单核苷酸多态性的分布,探讨该基因多态性位点与慢性肾脏病动脉硬化的相关性。方法:116例慢性肾脏病非透析患者均进行彩超测定颈动脉内膜厚度,并记录动脉硬化的传统危险因素,根据测定结果分为动脉硬化组(56例)和对照组(60例)。应用Taq Man探针等位基因特异性杂交分析法对Klotho基因G-395A多态性位点进行分析。结果:G-395A多态性位点共检测出GG、GA、AA 3种基因型,频率分别为56.9%、32.8%和10.3%,符合Hardy-Weinberg平衡。动脉硬化组G-395A等位基因的频率显著低于对照组(35.1%vs49.0%,P=0.032)。logistic回归分析,调整传统危险因素后G-395A与动脉硬化呈负相关(β=-0.412,P=0.024)。结论:Klotho基因G-395A等位基因可能是慢性肾脏病动脉硬化的遗传学保护因素。 Objective：To investigate the distribution of G - 395A polymorphism in promoter of Klotho gene in Chronic Kid-ney Disease Patients and analyze its association with arteriosclerosis. Methods：Genotyping of G - 395A polymorphism was performed in 56 arteriosclerotic patients of CKD and 60 controls using an allelic discrimination assay with TaqMan probes. Carotid artery intima- media thickness and atherosclerotic plaques were measured as a marker of atherosclerotic vascular damage. Results：Genotype fre-quencies of GG,GA and AA were 56. 9% . 32. 8% and10. 3% respectively. The distribution was in accordance with Hardy - Weinberg equilibrium. The frequency of the A allele carriers was signif - icantly lower in the arteriosclerosis group than in the control group （35. 1% vs 49. 0% ,P ﹤ 0. 05）. Logistic regression analysis revealed that after adjusted for traditional risk factors,the Aallele was＆amp;nbsp;negatively correlated with arteriosclerosis（β = - 0. 412,P ﹤ 0. 05）. Conclusion：The A allele of G - 395A polymorphism of Klotho gene may be a protective factor against arteriosclerosis in CKD patients.

作者陈金艳胡勇李慧赵玉刘丽秋

机构地区青岛大学附属海慈医院肾病科山东省青岛市海慈医疗集团干保科青岛大学附属医院

出处《中国中西医结合肾病杂志》 2015年第6期517-519,共3页 Chinese Journal of Integrated Traditional and Western Nephrology

关键词慢性肾脏病动脉硬化多态性单核苷酸启动区(遗传学) Chronic kidney disease Arteriosclerosis Polymorphism Single nucleotide

分类号 R692 [医药卫生—泌尿科学]

引文网络
相关文献

参考文献14

1Meyer KB, Levey AS. Controlling the epidemic of cardiovascular disease in chronic renal disease:report from the national kidney foundation task force on cardiovascular disease. J Am Soc Neph- ro1,1998,9(12 Suppl) :$31 -$42.
2Kato Y, Arakawa E, Kinoshita S, et al. Establishment of the an- tiKlotbo monoclonal antibodies and detection of Klotho protein in kidneys. Biochem Biophys Res Commun ,2000,267 (2) :597 - 602.
3Krajisnik T, Olauson H, Mirza MA, et al. Parathyroid Klotho and FGF - receptor 1 expression decline with renal function in hyper- parathyroid patients with chronic kidney disease and kidney transplant recipients. Kidney Int,2010,78 (10) : 1024 - 1032.
4Hu MC, Shi M, Zhang J, et al. Klotho deficiency causes vascular calcification in chronic kidney disease. J Am Soc Nephrol,2011, 22(1) :124 -136.
5Kawano K, Ogata N, Chiano M, et al. Klotho gene polymorphisms associated with bone density of aged postmenopausal women. J Bone Miner Res,2002,17 (10) : 1744 - 1751.
6Imamura A, Okumura K, Ogawa Y, et al. Klotho gene polymor- phism may be a genetic risk factor for atherosclerotic coronary ar- tery disease but not for vasospastic angina in Japanese. Clin Chim Aeta,2006,371 ( 1 - 2) :66 - 70.
7Kurosu H, Ogawa Y, Miyoshi M, et al. Regulation of fibroblast growth factor- 23 signaling by Klotho. J Biol Chem,2006,281 (10) :6120-6123.
8Rhee E J, Oh KW, Yun E J, et morphisms G395A in promoter al. Relationship between poly- and C1818T in exon 4 of the KLOTHO gene with glucose metabolism and cardiovascular risk fac- tors in Korean women. J Endoerinol Invest,2IX}6,29(7) :613 -618.
9Kim Y, Kim JH, Nam Y J, et al. Klotho is a genetic risk factor for isehemic stroke caused by cardioembolism in Korean females. Neurosci Lett, 2006,407 ( 3 ) : 189 - 194.
10Rhe E J, Oh KW, Lee WY, et al. The differential effects of age on the association of KLOTHO gene polymorphisms with coro- nary artery disease. Metabolism,2006,55 (10) :1344 - 1351.