ICSI助孕患者滤泡液中T、B淋巴细胞亚群对获卵率的影响

Impact of T and B lymphocyte subsets in follicular fluid on oocyte retrieved rate in patients with ICSI

目的通过测定卵胞浆内单精子注射(ICSI)助孕患者滤泡液中T、B淋巴细胞亚群来探讨T、B淋巴细胞亚群对获卵率的影响。方法前瞻性研究ICSI助孕的患者40例,根据获卵率的多少分为获卵正常组(获卵率>80%,A组,n=20)与获卵差组(获卵率<50%,B组,n=20),采用流式细胞仪对两组患者滤泡液中T、B淋巴细胞亚群进行分析,探讨其相关性。结果 A组的平均获卵数、MⅡ卵数、受精卵数及可利用胚胎数均显著高于B组(P<0.05),获卵率(90.8%vs.48.9%)亦显著高于B组(P<0.05)。B组患者滤泡液中CD8+T淋巴细胞[(21.09±4.55)%]显著高于A组[(10.35±3.21)%](P<0.05);两组患者滤泡液中CD4+T细胞数[(65.57±12.35)%vs.(62.34±11.62)%]及B淋巴细胞数[(4.78±1.69)%vs.(6.08±2.47)%]比较,差异无统计学意义(P>0.05)。结论滤泡液中CD8+T细胞的比例增高可能影响到ICSI助孕患者的获卵率。

Objective： To investigate the impact of T and B lymphocyte subsets in follicular fluid（FF）on the rate of oocyte retrieved in infertile women with ICSI treatment. Methods： Forty ICSI patients were divided into two groups prospectively according the rate of oocyte retrieved：group A（oocyte retrieved rate more than 80 %, n= 20）and group B（oocyte retrieved rate less than 50%,n= 20）. The T and B lymphocyte subsets in FF were analyzed by the flow cytometer. The correlation between T and B lymphocyte subsets and the rate of oocyte retrieved was analyzed. Results： The oocyte rate（90.8%）of group A was significantly higher than group B（48.9%）（P〈 0.05）. The percentage of CD8＋T cell in FF of group B was significantly higher than that of group A（P〈0. 05）,while there was no significant difference in CD4＋T and B lymphocyte cells in FF between the two groups（P〈0.05）. Conclusions： A higher percentage of CD8＋T cell in FF may affect the rate of oocyte retrieved in infertile women with ICSI treatment.