人胃癌组织中人巨噬细胞金属弹力酶、单核细胞趋化蛋白-1的表达及临床意义

Clinical significance of expression of human macrophage metalloelastase and monocyte chemoattractant protein-1 in gastric cancer

目的:研究人巨噬细胞金属弹力酶(human macrophage metalloelastase,HME)和单核细胞趋化蛋白-1(monocyte chemoattractant protein-1,MCP-1)在胃癌组织中的表达及临床意义.方法:应用免疫组织化学SP法检测胃癌和癌旁组织石蜡包埋组织标本30例中的HME和MCP-1蛋白的表达情况,并分析HME与MCP-1的相关性及两者与临床病理的关系.结果:(1)HME和MCP-1在胃癌组织中表达的阳性率为70.0%(21/30)和80.0%(24/30),而在癌旁组织中表达的阳性率为26.7%(8/30)和16.7%(5/30),二者在胃癌组织中的表达显著高于胃正常组织,差异有统计学意义(P<0.05);(2)HME在胃癌组织中的阳性表达与胃癌的分化程度,血管浸润有关(P<0.05),与其他临床病理参数无明显相关;MCP-1的表达与患者的肿瘤的浸润深度、肿瘤大小、肿瘤分期、淋巴转移、远处转移有关(P<0.05),与其他临床病理参数无明显相关;(3)HME与MCP-1在胃癌组织中的阳性表达无相关性(列联分析r=0.115,P>0.05).结论:胃癌组织中HME、MCP-1的高表达与胃癌的血管生成、浸润、转移有关,参与人胃癌的发生和发展,有助于探讨胃癌的发生机制.

AIM：To investigate the expression of human macrophage metalloelastase（HME） and monocyte chemoattractant protein-1（MCP-1）in gastric cancer and to analyze their clinical significance.METHODS：The expression of HME and MCP-1proteins in 30 specimens of gastric carcinoma and matched normal tissues was detected by immunohistochemical SP method.The correlation of HME and MCP-1 expression and their relationship with clinicopathologic features were analyzed.RESULTS：In gastric carcinoma,the positive rates of HME and MCP-1 expression were70.0%（21/30） and 80.0%（24/30）,respectively,which were significantly higher than those in normal gastric tissues[26.7%（8/30） and16.7%（5/30）]（P〈0.05）.The expression of HME was significantly associated with degree of differentiation and blood vessel invasion.The expression of MCP-1 was significantly associated with depth of invasion,tumor size,tumor stage and lymph node and distant metastasis.The expression of HME was not correlated with that of MCP-1 in gastric carcinoma（r = 0.115,P〉0.01）.CONCLUSION：HME and MCP-1 are positively expressed in gastric carcinoma,and there is no correlation between HME and MCP-1expression.Their expression may be closely related with the tumor angiogenesis,infiltration and metastasis in gastric cancer.