饮用水有机提取物对大鼠氧化应激及免疫功能的影响

Effect of Organic Extracts from Drinking Water on Rats' Oxidative Stress and Immune Function

目的:探讨某地饮用水有机提取物对大鼠氧化应激及免疫功能的影响,为当地饮用水卫生学评价及人群健康保护提供依据。方法:采用固相萃取法提取水样中有机污染物,40只SD大鼠随机均分成4组,对照组给予玉米油、高中低剂量组给予80、20及5 L/kg有机污染进行灌胃12周后,取小鼠血清和肝匀浆,分别采用黄嘌呤氧比酶法和硫化巴比妥法测定超氧化物歧化酶(SOD)、丙二醛(MDA)的活性与含量;微量酶标法检测谷胱甘肽过氧化物酶(GSH-Px)的活性;透射比浊法检测血青中免疫球蛋白(Ig M、Ig G、Ig A)与补体(C3、C4)的含量。结果:与对照组相比,血清SOD活力在低剂量组明显升高、高剂量组明显降低,GSH-Px活力在高剂量组明显下降,MDA含量在中、高剂量组均明显升高;肝组织SOD活力仅在高剂量组降低,GSH-Px活力则在中、高剂量组都显著降低,MDA含量在中、高剂量组均明显升高,差异均具有统计学意义(P<0.05);随着染毒剂量的增加,中、高剂量组的Ig M含量较对照组明显升高,差异有统计学意义(P<0.05);Ig A、Ig G、C3及C4则无明显改变,差异无统计学意义(P>0.05)。结论:该地较高剂量的饮用水有机污染物可诱导大鼠肝脏氧化损伤的发生,并可诱导机体的初次免疫应答增强,而对机体的再次免疫应答及非特异性免疫无明显诱导作用。

Objective： To explore the oxidative stress and immune function in rats exposed to organic extracts from drinking water and provide the scientific basis for hygienic evaluation of drinking water and health protection in G city. Methods： Organic pollutants in drinking water were extracted by solid - phase extraction method, which were dissolved in corn oil and made as 50 L/mL solution of organic pollutants. 40 SD rats were randomly divided into 4 groups： corn oil group （control group）, high dose group （80L/kg）, middle dose group （20 l_,/kg）, low dose group （5 L/kg）. The contamination expo- sure lasted 12 weeks by intragastric administration. The activities and content of SOD, MDA were measured by spectrophotometry. Trace enzyme labelling was adopted to measure the GSH - Px activity. Transmission turbidity method was used to detect the content of immunoglobulin （IgM, IgG, IgA） and complement （C3, C4）. Results： Compared with control group, the enzyme activity of serum SOD in low dose group obviously increased while in high dose group significantly decreased. Serum GSH -Px in high dose group obviously decreased. The MDA content in serum significantly increased in middle and high dose group. In high dose group, SOD activity in liver tissue was significantly decreased. GSH - Px activity of liver tissue in middle dose, high dose groups was significantly decreased. The MDA content in liver tissue significantly increased in middle and high dose group, and the difference was statistically significant （P 〈 0.05 ）. With the rise of exposure dose, the level of IgM in middle dose and high dose group increased obviously compared with control group （P 〈 0.05 ） while the level of IgA, IgG, C3 and C4 showed no significant change （P 〉 0.05）. Conclusion：The higher dose of organ- ic pollutants in drinking water can induce oxidative body, but have no obvious effect on secondary damage of liver and primary immune response of immune response and non -specific immunity